Abstract：
OBJECTIVE: To evaluate temporal changes in serum C-reactive protein (CRP) and haptoglobin (Hp) concentrations in dogs with pulmonary coccidioidomycosis and assess their utility to detect remission.
METHODS: 31 client-owned dogs with newly diagnosed pulmonary coccidioidomycosis from October 2020 to February 2021 were included in a retrospective cohort study that utilized archived serum. Serum was originally obtained at diagnosis and once every 3 months after antifungal administration until either remission or 12 months. Time points were designated as baseline (T0), 3 months (T1), 6 months (T2), 9 months (T3), and 12 months (T4). Serum CRP and Hp were measured at a reference laboratory with ELISA assays.
RESULTS: Median serum CRP and Hp concentrations decreased from T0 (CRP, 56 mg/L; Hp, 716.1 mg/dL) to T1 (CRP, 3.3 mg/L; Hp, 240.5 mg/dL); subsequent decreases were not significant. Eighteen (60%) and 16 (53%) of 30 dogs had normal serum CRP and Hp concentrations at T1, respectively. Absolute serum CRP (AUC, 0.58; 95% CI, 0.45 to 0.72) and Hp (AUC, 0.65; 95% CI, 0.52 to 0.78) were poor detectors of remission. However, the percentage change in Hp from T0 to T1 (AUC, 0.90; 95% CI, 0.74 to 1.0) was an excellent predictor of remission within 12 months.
CONCLUSIONS: Serum CRP and Hp concentrations decrease in the first 3 months of antifungal treatment in dogs with pulmonary coccidioidomycosis, and the percentage change of Hp may help predict dogs that will achieve remission within 12 months of treatment.
CONCLUSIONS: Serum CRP and Hp may be useful adjunctive biomarkers to monitor treatment response in dogs with pulmonary coccidioidomycosis.
METHODS: 31 client-owned dogs with newly diagnosed pulmonary coccidioidomycosis from October 2020 to February 2021 were included in a retrospective cohort study that utilized archived serum. Serum was originally obtained at diagnosis and once every 3 months after antifungal administration until either remission or 12 months. Time points were designated as baseline (T0), 3 months (T1), 6 months (T2), 9 months (T3), and 12 months (T4). Serum CRP and Hp were measured at a reference laboratory with ELISA assays.
RESULTS: Median serum CRP and Hp concentrations decreased from T0 (CRP, 56 mg/L; Hp, 716.1 mg/dL) to T1 (CRP, 3.3 mg/L; Hp, 240.5 mg/dL); subsequent decreases were not significant. Eighteen (60%) and 16 (53%) of 30 dogs had normal serum CRP and Hp concentrations at T1, respectively. Absolute serum CRP (AUC, 0.58; 95% CI, 0.45 to 0.72) and Hp (AUC, 0.65; 95% CI, 0.52 to 0.78) were poor detectors of remission. However, the percentage change in Hp from T0 to T1 (AUC, 0.90; 95% CI, 0.74 to 1.0) was an excellent predictor of remission within 12 months.
CONCLUSIONS: Serum CRP and Hp concentrations decrease in the first 3 months of antifungal treatment in dogs with pulmonary coccidioidomycosis, and the percentage change of Hp may help predict dogs that will achieve remission within 12 months of treatment.
CONCLUSIONS: Serum CRP and Hp may be useful adjunctive biomarkers to monitor treatment response in dogs with pulmonary coccidioidomycosis.
摘要：
目的：评估肺球孢子菌病犬血清C反应蛋白（CRP）和结合珠蛋白（Hp）浓度的时间变化，并评估其在检测缓解中的作用。
方法：将2020年10月至2021年2月的31只新诊断的肺球孢子菌病患者纳入一项利用存档血清的回顾性队列研究。最初在诊断时获得血清，并在抗真菌药施用后每3个月获得一次，直到缓解或12个月。时间点被指定为基线（T0），3个月（T1），6个月（T2），9个月（T3），12个月（T4）。在参考实验室用ELISA测定法测量血清CRP和Hp。
结果：血清CRP和Hp浓度中位数从T0（CRP，56mg/L；Hp，716.1mg/dL）至T1（CRP，3.3mg/L;Hp,240.5mg/dL）；随后的下降并不显著。30只狗中的18只（60％）和16只（53％）分别在T1时具有正常的血清CRP和Hp浓度。绝对血清CRP（AUC，0.58；95%CI，0.45至0.72）和Hp（AUC，0.65；95%CI，0.52至0.78）是缓解不良的检测指标。然而,Hp从T0到T1的百分比变化(AUC，0.90；95％CI，0.74至1.0）是12个月内缓解的良好预测指标。
结论：肺球孢子菌病犬抗真菌治疗前3个月血清CRP和Hp浓度下降，和Hp的百分比变化可能有助于预测在治疗后12个月内达到缓解的狗。
结论：血清CRP和Hp可能是监测肺球孢子菌病犬治疗反应的有用辅助生物标志物。
方法：将2020年10月至2021年2月的31只新诊断的肺球孢子菌病患者纳入一项利用存档血清的回顾性队列研究。最初在诊断时获得血清，并在抗真菌药施用后每3个月获得一次，直到缓解或12个月。时间点被指定为基线（T0），3个月（T1），6个月（T2），9个月（T3），12个月（T4）。在参考实验室用ELISA测定法测量血清CRP和Hp。
结果：血清CRP和Hp浓度中位数从T0（CRP，56mg/L；Hp，716.1mg/dL）至T1（CRP，3.3mg/L;Hp,240.5mg/dL）；随后的下降并不显著。30只狗中的18只（60％）和16只（53％）分别在T1时具有正常的血清CRP和Hp浓度。绝对血清CRP（AUC，0.58；95%CI，0.45至0.72）和Hp（AUC，0.65；95%CI，0.52至0.78）是缓解不良的检测指标。然而,Hp从T0到T1的百分比变化(AUC，0.90；95％CI，0.74至1.0）是12个月内缓解的良好预测指标。
结论：肺球孢子菌病犬抗真菌治疗前3个月血清CRP和Hp浓度下降，和Hp的百分比变化可能有助于预测在治疗后12个月内达到缓解的狗。
结论：血清CRP和Hp可能是监测肺球孢子菌病犬治疗反应的有用辅助生物标志物。
