Abstract：
The Hippophae rhamnoides L. pomace was generated in the production process for juice, wine of food industry. To expand the application of pomace, the extraction process optimization, enrichment and identification of triterpene acids were performed in this study. The extraction yield was 14.87% under optimal ultrasound-assisted extraction techniques performed via response surface methodology. The extract was subsequently purified to obtain the triterpenoid acid enrichment fraction (TPF) with the content of 75.23% ± 1.45%. 13 triterpenoid acids were identified via UPLC-Triple-TOF MS/MS and further semi-quantified through comparison with triterpenoid acid standards. TPF exhibited a strong inhibitory effect on α-glucosidase with IC50 value of 5.027 ± 0.375 μg/mL, as determined via enzyme inhibition experiment and molecular docking. Additionally, the TPF significantly reduced postprandial glucose levels, as revealed via carbohydrate tolerance tests, as well as ameliorate serum lipid profiles. Therefore, pomace may be a promising resource of functional food components with therapeutic and commercial values.
摘要：
沙棘果渣是在果汁生产过程中产生的，食品工业的葡萄酒。为了扩大果渣的应用范围，提取工艺优化,对三萜酸进行了富集和鉴定。在通过响应面方法进行的最佳超声辅助提取技术下，提取率为14.87％。随后纯化提取物以获得含量为75.23%±1.45%的三萜酸富集级分(TPF)。通过UPLC-Triple-TOFMS/MS鉴定13种三萜酸,并通过与三萜酸标准品比较进一步半定量。TPF对α-葡萄糖苷酶有很强的抑制作用,IC50值为5.027±0.375μg/mL,通过酶抑制实验和分子对接确定。此外,TPF显着降低餐后葡萄糖水平，正如通过碳水化合物耐受性测试所揭示的，以及改善血清脂质状况。因此,果渣可能是具有治疗和商业价值的功能性食品成分的有希望的资源。
