Abstract：
Anti-Müllerian hormone (AMH) serves as an indirect marker for predicting primordial follicles that are representative of ovarian reserve. In this study the possibility of using AMH and age to predict the ovarian reserve in domestic cats. Ovaries and blood were collected from 30 cats undergoing routine ovariohysterectomy. The animals were divided into three age groups: prepubertal (<4 mo, n = 10), adult (1-5 y, n = 10), and senior (>5 y, n = 10). Blood was collected at surgery for serum AMH measurements using the AMH Gen II ELISA kit. The intra-assay coefficient of variation (CV) and inter-assay CV were 3.56 % and 7.68 %, respectively. One side of the ovary was processed to determine AMH localization using immunohistochemistry and for a histological count of follicles, which is the gold standard. The expression of AMH protein was quantified from the contralateral ovary by Western blot analysis. Primordial follicles exhibited the most pronounced inverse relationship with age (rho = -0.779, P < 0.05), followed by a positive association with serum AMH concentration (rho = 0.490, P < 0.05), indicating that both age and AMH are potential markers indicative of primordial follicles. Furthermore, secondary (rho = 0.651, P < 0.05) and small antral follicles (rho = 0.648, P < 0.05) were identified as the major sources of circulating AMH, as indicated by the stronger correlation with serum AMH concentrations compared with primary follicles. However, there was no significant correlation between the expression of AMH protein and other factors, including age, primordial follicles, primary follicles, secondary follicles, small antral follicles, and serum AMH concentration. A model for predicting primordial follicle number using serum AMH concentration (AIC = 672.66, P < 0.05) and age (AIC = 668.93, P < 0.05) was established. In conclusion, both serum AMH concentration and age may serve as comparable markers of ovarian reserve in domestic cats. Moreover, AMH is particularly useful in situations where age information is not available.
摘要：
抗苗勒管激素（AMH）是预测代表卵巢储备的原始卵泡的间接标志物。在这项研究中，使用AMH和年龄来预测家猫卵巢储备的可能性。从进行常规卵巢子宫切除术的30只猫收集卵巢和血液。将动物分为三个年龄组：青春期前（<4个月，n=10),成人（1-5岁，n=10),和高年级(>5岁，n=10)。在手术时收集血液用于使用AMHGenIIELISA试剂盒进行血清AMH测量。试验内变异系数(CV)和试验间变异系数分别为3.56%和7.68%,分别。对卵巢的一侧进行处理以使用免疫组织化学和卵泡的组织学计数来确定AMH定位。这是黄金标准。通过Western印迹分析从对侧卵巢定量AMH蛋白的表达。原始卵泡与年龄成反比关系最明显(rho=-0.779,P<0.05),与血清AMH浓度呈正相关(rho=0.490,P<0.05),表明年龄和AMH都是指示原始卵泡的潜在标志物。此外，次级（rho=0.651，P<0.05）和小的窦卵泡（rho=0.648，P<0.05）被确定为循环AMH的主要来源，与初级卵泡相比，与血清AMH浓度的相关性更强。然而,AMH蛋白的表达与其他因素无显著相关性,包括年龄,原始卵泡,初级卵泡,次级卵泡,小的窦卵泡,和血清AMH浓度。建立血清AMH浓度(AIC=672.66,P<0.05)和年龄(AIC=668.93,P<0.05)预测原始卵泡数的模型。总之,血清AMH浓度和年龄均可作为家猫卵巢储备的可比标志。此外，AMH在没有年龄信息的情况下特别有用。
