Abstract：
A genome-based polyphasic approach was used to determine the taxonomic status of two novel bacterial strains, SCSIO 12594T and SCSIO 12813T, isolated from tissues of a coral. Both strains were Gram-stain-negative and facultatively anaerobic. The genome sizes of strains SCSIO 12594T and SCSIO 12813T were 3.9 Mb and 4.1 Mb, respectively, and they possessed DNA G+C contents of 55.1 and 46.2 mol%, respectively . Both strains were found to be catalase- and oxidase-positive, while SCSIO 12594T also could hydrolyse starch. SCSIO 12594T was observed to grow at between 20 and 37 °C (optimally at 25 °C) and at a pH range from 6 to 7 and in the presence of 3-7 % (w/v) NaCl. The growth of SCSIO 12813T required seawater and occurred at 20-30 °C (optimum, 25 °C), pH 5-8 (optimum, pH 6-7) and in the presence of 3-3.7 % (w/v) NaCl. The results of 16S rRNA gene-based phylogenetic analysis indicated that SCSIO 12594T shared 92.97 % or less sequence similarity with its closest relatives Rhodobium gokarnense JA173T and other members of the order Hyphomicrobiales. The results of 16S rRNA sequences-based phylogenetic analysis of SCSIO 12813T indicated that Croceimicrobium hydrocarbonivorans A20-9T (89.34 %) was the most closely related species. SCSIO 12594T and SCSIO 12813T can be readily separated from their closest relatives, as indicated by the results of phylogenomic analysis, low average nucleotide indexes, average amino acid identity, digital DNA-DNA hybridisation (dDDH) similarities and associated phenotypic and chemical data. Consequently, the two coral isolates are considered to represent two novel genera and species for which the names Coralliovum pocilloporae gen. nov., sp. nov. and Sanyastnella coralliicola gen. nov., sp. nov. are proposed, the type strains are SCSIO 12594T (= JCM 35320T = GDMCC 1.3060T) and SCSIO 12813T (= JCM 35373T = GDMCC 1.3063T), respectively. In addition, two novel families, Coralliovaceae fam. nov. and Sanyastnellaceae fam. nov are proposed to accommodate Coralliovum pocilloporae gen. nov., sp. nov. and Sanyastnella coralliicola gen. nov., sp. nov., respectively.
摘要：
基于基因组的多相方法用于确定两个新的细菌菌株的分类状态，SCSIO12594T和SCSIO12813T,从珊瑚的组织中分离出来。两种菌株均为革兰氏染色阴性且兼性厌氧。菌株SCSIO12594T和SCSIO12813T的基因组大小分别为3.9Mb和4.1Mb,分别,它们的DNAGC含量为55.1和46.2mol%，分别。发现两种菌株都是过氧化氢酶和氧化酶阳性，而SCSIO12594T也能水解淀粉。观察到SCSIO12594T在20至37°C（最佳在25°C）和6至7的pH范围内以及在3-7％（w/v）NaCl存在下生长。SCSIO12813T的生长需要海水，并且发生在20-30°C（最佳，25°C),pH5-8（最佳，pH6-7）和在3-3.7％（w/v）NaCl存在下。基于16SrRNA基因的系统发育分析结果表明，SCSIO12594T与其最接近的近亲RhodobiumgokarnenseJA173T和其他细菌菌目成员具有92.97％或更低的序列相似性。对SCSIO12813T进行基于16SrRNA序列的系统发育分析的结果表明，碳食草杆菌A20-9T（89.34％）是最密切相关的物种。SCSIO12594T和SCSIO12813T可以很容易地与他们的近亲分开，正如系统发育分析的结果表明，低平均核苷酸指数，平均氨基酸同一性，数字DNA-DNA杂交（dDDH）相似性和相关的表型和化学数据。因此,这两个珊瑚分离株被认为代表了两个新的属和种，其名称为珊瑚属。11月。,sp.11月。还有Sanyastnellacoralliicolagen.11月。,sp.11月。被提议，菌株类型为SCSIO12594T（=JCM35320T=GDMCC1.3060T）和SCSIO12813T（=JCM35373T=GDMCC1.3063T），分别。此外,两个小说家庭，珊瑚科。11月。和三叶草科fam。建议将nov容纳小科珊瑚。11月。,sp.11月。还有Sanyastnellacoralliicolagen.11月。,sp.11月。,分别。
