Abstract：
Sessile organisms, such as plants, developed various ways to sense and respond to external and internal stimuli to maximize their fitness through evolutionary time. Transcripts and protein regulation are, among many, the main mechanisms that plants use to respond to environmental changes. SKIP protein is one such, presenting an SNKW interacting domain, which is highly conserved among eukaryotes, where SKI interacting protein acts in regulating key processes. In the present work, many bioinformatics tools, such as phylogenetic relationships, gene structure, physical-chemical properties, conserved motifs, prediction of regulatory cis-elements, chromosomal localization, and protein-protein interaction network, were used to better understand the genome-wide SNW/SKIP domain-containing proteins. In total, 28 proteins containing the SNW/SKIP domain were identified in different plant species, including plants of agronomic interest. Two main protein clusters were formed in phylogenetic analysis, and gene structure analysis revealed that, in general, the coding region had no introns. Also, expression of these genes is possibly induced by abiotic stress stimuli. Primary structure analysis of the proteins revealed the existence of an evolutionarily conserved functional unit. But physicochemical properties show that proteins containing the SNW/SKIP domain are commonly unstable under in vivo conditions. In addition, the protein network, demonstrated that SKIP homologues could act by modulating plant fitness through gene expression regulation at the transcriptional and post-transcriptional levels. This could be corroborated by the expression number of gene copies of SKIP proteins in many species, highlighting it\'s crucial role in plant development and tolerance through the course of evolution.
摘要：
静止生物，比如植物,开发了各种方法来感知和响应外部和内部刺激，以通过进化时间最大化它们的适应性。转录本和蛋白质调节是，在许多人中,植物用来应对环境变化的主要机制。SKIP蛋白就是这样一种，呈现SNKW交互域，在真核生物中高度保守，其中SKI相互作用蛋白在调节关键过程中起作用。在目前的工作中,许多生物信息学工具，比如系统发育关系，基因结构，物理化学性质，保守的图案，调节顺式元素的预测，染色体定位，和蛋白质-蛋白质相互作用网络，用于更好地了解全基因组的含SNW/SKIP结构域的蛋白质。总的来说,在不同的植物物种中鉴定出28种含有SNW/SKIP结构域的蛋白质，包括具有农艺意义的植物。在系统发育分析中形成了两个主要的蛋白质簇，基因结构分析表明，总的来说,编码区没有内含子.此外，这些基因的表达可能是由非生物应激刺激诱导的。蛋白质的一级结构分析揭示了进化上保守的功能单元的存在。但是物理化学性质表明,含有SNW/SKIP结构域的蛋白质在体内条件下通常是不稳定的。此外,蛋白质网络，证明SKIP同源物可以通过转录和转录后水平的基因表达调节来调节植物的适应性。这可以通过许多物种中SKIP蛋白的基因拷贝数来证实，强调它在植物进化过程中的发育和耐受性的关键作用。
