Abstract：
BACKGROUND: Pasteurized human donor milk (DM) is frequently used for feeding preterm newborns and extrauterine growth-restricted (EUGR) infants. Most human milk banks performed a pasteurization of DM using the standard method of Holder pasteurization (HoP) which consists of heating milk at 62.5°C for 30 min. High hydrostatic pressure (HHP) processing was proposed to be an innovative nonthermal method to pasteurize DM. However, the effect of different modes of DM pasteurization on body growth, intestinal maturation, and microbiota has never been investigated in vivo during the lactation.
OBJECTIVE: We aimed to study these effects in postnatally growth-restricted (PNGR) mice pups daily supplemented with HoP-DM or HHP-DM.
METHODS: PNGR was induced by increasing the number of pups per litter (15 pups/mother) at postnatal Day 4 (PND4). From PND8 to PND20, mice pups were supplemented with HoP-DM or HHP-DM. At PND21, the intestinal permeability was measured in vivo, the intestinal mucosal histology, gut microbiota, and short-chain fatty acids (SCFAs) level were analyzed.
RESULTS: HHP-DM pups displayed a significantly higher body weight gain than HoP-DM pups during lactation. At PND21, these two types of human milk supplementations did not differentially alter intestinal morphology and permeability, the gene-expression level of several mucosal intestinal markers, gut microbiota, and the caecal SCFAs level.
CONCLUSIONS: Our data suggest that HHP could be an attractive alternative to HoP and that HHP-DM may ensure a better body growth of preterm and/or EUGR infants.
OBJECTIVE: We aimed to study these effects in postnatally growth-restricted (PNGR) mice pups daily supplemented with HoP-DM or HHP-DM.
METHODS: PNGR was induced by increasing the number of pups per litter (15 pups/mother) at postnatal Day 4 (PND4). From PND8 to PND20, mice pups were supplemented with HoP-DM or HHP-DM. At PND21, the intestinal permeability was measured in vivo, the intestinal mucosal histology, gut microbiota, and short-chain fatty acids (SCFAs) level were analyzed.
RESULTS: HHP-DM pups displayed a significantly higher body weight gain than HoP-DM pups during lactation. At PND21, these two types of human milk supplementations did not differentially alter intestinal morphology and permeability, the gene-expression level of several mucosal intestinal markers, gut microbiota, and the caecal SCFAs level.
CONCLUSIONS: Our data suggest that HHP could be an attractive alternative to HoP and that HHP-DM may ensure a better body growth of preterm and/or EUGR infants.
摘要：
背景：巴氏灭菌的人供体乳（DM）经常用于喂养早产新生儿和宫外生长受限（EUGR）婴儿。大多数人乳库使用Holder巴氏灭菌法（HoP）的标准方法对DM进行巴氏灭菌，该方法包括在62.5°C下加热牛奶30分钟。提出了高静水压力（HHP）处理是一种创新的非热巴氏杀菌方法。然而,不同的DM巴氏杀菌模式对机体生长的影响，肠道成熟，泌乳期间从未在体内研究过微生物群。
目的：我们旨在研究在每天补充HoP-DM或HHP-DM的出生后生长受限（PNGR）小鼠幼崽中的这些作用。
方法：通过在出生后第4天（PND4）增加每窝幼仔的数量（15幼仔/母亲）来诱导PNGR。从PND8到PND20,小鼠幼崽补充有HoP-DM或HHP-DM。在PND21，在体内测量肠通透性，肠粘膜组织学，肠道菌群，短链脂肪酸（SCFA）水平进行分析。
结果：在哺乳期，HHP-DM幼崽的体重增加明显高于HoP-DM幼崽。在PND21，这两种类型的人乳补充剂没有差异改变肠道形态和通透性，几种粘膜肠道标志物的基因表达水平，肠道菌群，和盲肠SCFA水平。
结论：我们的数据表明HHP可能是HHP的一种有吸引力的替代方案，HHP-DM可以确保早产和/或EUGR婴儿更好的身体生长。
目的：我们旨在研究在每天补充HoP-DM或HHP-DM的出生后生长受限（PNGR）小鼠幼崽中的这些作用。
方法：通过在出生后第4天（PND4）增加每窝幼仔的数量（15幼仔/母亲）来诱导PNGR。从PND8到PND20,小鼠幼崽补充有HoP-DM或HHP-DM。在PND21，在体内测量肠通透性，肠粘膜组织学，肠道菌群，短链脂肪酸（SCFA）水平进行分析。
结果：在哺乳期，HHP-DM幼崽的体重增加明显高于HoP-DM幼崽。在PND21，这两种类型的人乳补充剂没有差异改变肠道形态和通透性，几种粘膜肠道标志物的基因表达水平，肠道菌群，和盲肠SCFA水平。
结论：我们的数据表明HHP可能是HHP的一种有吸引力的替代方案，HHP-DM可以确保早产和/或EUGR婴儿更好的身体生长。
