PDF(Pubmed)
Abstract：
The vaginal microbiome plays an essential role in the reproductive health of human females. As infertility increases worldwide, understanding the roles that the vaginal microbiome may have in infertility and in vitro fertilization (IVF) treatment outcomes is critical. To determine the vaginal microbiome composition of 1411 individuals (1255 undergoing embryo transplantation) and their associations with reproductive outcomes, clinical and biochemical features are measured, and vaginal samples are 16S rRNA sequenced. Our results suggest that both too high and too low abundance of Lactobacillus is not beneficial for pregnancy; a moderate abundance is more beneficial. A moderate abundance of Lactobacillus crispatus and Lactobacillus iners (~80%) (with a pregnancy rate of I-B: 54.35% and III-B: 57.73%) is found beneficial for pregnancy outcomes compared with a higher abundance (>90%) of Lactobacillus (I-A: 44.81% and III-A: 51.06%, respectively). The community state type (CST) IV-B (contains a high to moderate relative abundance of Gardnerella vaginalis) shows a similar pregnant ratio (48.09%) with I-A and III-A, and the pregnant women in this CST have a higher abundance of Lactobacillus species. Metagenome analysis of 71 samples shows that nonpregnant women are detected with more antibiotic-resistance genes, and Proteobacteria and Firmicutes are the main hosts. The inherent differences within and between women in different infertility groups suggest that vaginal microbes might be used to detect infertility and potentially improve IVF outcomes.
摘要：
阴道微生物组在人类女性的生殖健康中起着至关重要的作用。随着世界范围内不孕症的增加，了解阴道微生物组可能在不孕症和体外受精(IVF)治疗结局中的作用至关重要.为了确定1411名个体（接受胚胎移植的1255名）的阴道微生物组组成及其与生殖结果的关系，测量临床和生化特征，和阴道样品进行16SrRNA测序。我们的结果表明，过高和过低的乳酸菌丰度对怀孕都没有好处；适度的丰度更有益。发现中等丰度的卷曲乳杆菌和乳杆菌（〜80％）（妊娠率I-B：54.35％和III-B：57.73％）与更高丰度（>90％）的乳杆菌（I-A：44.81％和III-A：51.06％，分别)。群落状态类型（CST）IV-B（含有高至中等相对丰度的阴道加德纳菌）与I-A和III-A的妊娠率相似（48.09％），在这个CST中，孕妇的乳杆菌属物种的丰度更高。对71个样本的宏基因组分析表明，未怀孕的妇女被检测到具有更多的抗生素抗性基因，变形杆菌和厚壁槽是主要宿主。不同不孕症组女性内部和之间的固有差异表明，阴道微生物可能用于检测不孕症并可能改善IVF结局。
