RESULTS: Under high-temperature stress, proline (Pro), H2O2 content increased, then decreased, then increased again, catalase (CAT) activity increased continuously, peroxidase (POD) activity decreased rapidly, then increased, then decreased again, superoxide dismutase (SOD) activity, malondialdehyde (MDA), and soluble sugars (SS) content all decreased, then increased, and chlorophyll and soluble proteins (SP) content increased, then decreased. Transcriptomic investigation indicated that a total of 2740 DEGs were identified and numerous DEGs were notably enriched for \"Plant-pathogen interaction\" and \"Plant hormone signal transduction\". We identified a total of 32 genes in these two pathways that may be the key genes for resistance to high-temperature stress in A. roxburghii.
CONCLUSIONS: To sum up, the results of this study provide a reference for the molecular regulation of A. roxburghii\'s tolerance to high temperatures, which is useful for further cultivation of high-temperature-tolerant A. roxburghii varieties.
结果:在高温应力下,脯氨酸(Pro),H2O2含量增加,然后减少,然后再次增加,过氧化氢酶(CAT)活性持续增加,过氧化物酶(POD)活性迅速下降,然后增加,然后再次下降,超氧化物歧化酶(SOD)活性,丙二醛(MDA),可溶性糖(SS)含量均下降,然后增加,叶绿素和可溶性蛋白(SP)含量增加,然后减少。转录组学研究表明,总共鉴定了2740DEGs,并且许多DEGs在“植物-病原体相互作用”和“植物激素信号转导”方面都得到了显着富集。我们在这两个途径中总共鉴定了32个基因,它们可能是刺梨抗高温胁迫的关键基因。
结论:总而言之,本研究结果为刺梨耐高温的分子调控提供了参考,这对于进一步培育耐高温的刺梨品种是有用的。