Abstract：
OBJECTIVE: To evaluate the impact of high-intensity focused ultrasound (HIFU) ablation on the histopathological features of locally recurrent fibroids tissue.
METHODS: Patients who underwent transabdominal hysterectomy or myomectomy for uterine fibroids from January 1, 2021 to July 1, 2023 at a teaching hospital in China were enrolled in this prospective study. The patients who underwent surgery for local recurrence of uterine fibroids after HIFU ablation were categorized as the HIFU group, and patients who had not undergone HIFU ablation for uterine fibroids were the control group. Hematoxylin-eosin (HE) staining, Masson staining, and immunohistochemical staining were performed to analyze the counts of smooth muscle cells (SMCs), collagen content, microvascular count, and the expression levels of estrogen receptor (ER) and progesterone receptor (PR) in the fibroid tissue specimens.
RESULTS: The mean SMC counts in the HIFU and control groups were 337.68/field and 328.52/field respectively. The mean collagen content in the HIFU group and control group were 46.06% and 41.69% respectively. The mean microvessel counts in the HIFU group and control group were 13.66/field and 14.08/field respectively. The mean ER scores in the HIFU and control groups were 6.9 and 7.47 respectively, and the mean PR scores were 7.3 and 7.56 respectively. Overall, there were no significant differences in the SMC counts, collagen content, microvascular counts, and the ER and PR expression levels between the HIFU group and control group (p > 0.05).
CONCLUSIONS: HIFU ablation has no effect on the pathological characteristics of local recurrent fibroid tissue, and is an ideal non-invasive treatment option.
METHODS: Patients who underwent transabdominal hysterectomy or myomectomy for uterine fibroids from January 1, 2021 to July 1, 2023 at a teaching hospital in China were enrolled in this prospective study. The patients who underwent surgery for local recurrence of uterine fibroids after HIFU ablation were categorized as the HIFU group, and patients who had not undergone HIFU ablation for uterine fibroids were the control group. Hematoxylin-eosin (HE) staining, Masson staining, and immunohistochemical staining were performed to analyze the counts of smooth muscle cells (SMCs), collagen content, microvascular count, and the expression levels of estrogen receptor (ER) and progesterone receptor (PR) in the fibroid tissue specimens.
RESULTS: The mean SMC counts in the HIFU and control groups were 337.68/field and 328.52/field respectively. The mean collagen content in the HIFU group and control group were 46.06% and 41.69% respectively. The mean microvessel counts in the HIFU group and control group were 13.66/field and 14.08/field respectively. The mean ER scores in the HIFU and control groups were 6.9 and 7.47 respectively, and the mean PR scores were 7.3 and 7.56 respectively. Overall, there were no significant differences in the SMC counts, collagen content, microvascular counts, and the ER and PR expression levels between the HIFU group and control group (p > 0.05).
CONCLUSIONS: HIFU ablation has no effect on the pathological characteristics of local recurrent fibroid tissue, and is an ideal non-invasive treatment option.
摘要：
目的：评估高强度聚焦超声（HIFU）消融对局部复发性肌瘤组织的组织病理学特征的影响。
方法：这项前瞻性研究纳入了2021年1月1日至2023年7月1日在中国一家教学医院接受子宫肌瘤经腹子宫切除术或子宫肌瘤切除术的患者。将HIFU消融术后局部复发子宫肌瘤行手术的患者归为HIFU组。未进行子宫肌瘤HIFU消融术的患者为对照组。苏木精-伊红(HE)染色,Masson染色,和免疫组织化学染色分析平滑肌细胞（SMC）的计数，胶原蛋白含量，微血管计数,子宫肌瘤组织标本中雌激素受体(ER)和孕激素受体(PR)的表达水平。
结果：HIFU组和对照组的平均SMC计数分别为337.68/场和328.52/场。HIFU组和对照组的平均胶原含量分别为46.06%和41.69%。HIFU组和对照组的平均微血管计数分别为13.66/视野和14.08/视野。HIFU组和对照组的平均ER评分分别为6.9和7.47,平均PR评分分别为7.3和7.56。总的来说,SMC计数没有显着差异，胶原蛋白含量，微血管计数,HIFU组与对照组之间的ER和PR表达水平(p>0.05)。
结论：HIFU消融对局部复发性纤维瘤组织的病理特征无影响，是一种理想的非侵入性治疗选择。
方法：这项前瞻性研究纳入了2021年1月1日至2023年7月1日在中国一家教学医院接受子宫肌瘤经腹子宫切除术或子宫肌瘤切除术的患者。将HIFU消融术后局部复发子宫肌瘤行手术的患者归为HIFU组。未进行子宫肌瘤HIFU消融术的患者为对照组。苏木精-伊红(HE)染色,Masson染色,和免疫组织化学染色分析平滑肌细胞（SMC）的计数，胶原蛋白含量，微血管计数,子宫肌瘤组织标本中雌激素受体(ER)和孕激素受体(PR)的表达水平。
结果：HIFU组和对照组的平均SMC计数分别为337.68/场和328.52/场。HIFU组和对照组的平均胶原含量分别为46.06%和41.69%。HIFU组和对照组的平均微血管计数分别为13.66/视野和14.08/视野。HIFU组和对照组的平均ER评分分别为6.9和7.47,平均PR评分分别为7.3和7.56。总的来说,SMC计数没有显着差异，胶原蛋白含量，微血管计数,HIFU组与对照组之间的ER和PR表达水平(p>0.05)。
结论：HIFU消融对局部复发性纤维瘤组织的病理特征无影响，是一种理想的非侵入性治疗选择。
