Abstract：
OBJECTIVE: to investigate whether baseline mineral distribution modulates the ability of silver diammine fluoride (SDF) to remineralize and stain enamel caries lesions.
METHODS: This laboratory study followed a 3 [treatment: SDF/fluoride varnish (FV)/deionized water (DIW)] ×3 [lesion protocol: methylcellulose (MeC)/hydroxyethylcellulose (HEC)/Carbopol 907 (C907)] factorial design. Lesions were created in bovine enamel specimens (n = 20). Treatments were applied and lesions remineralized in artificial saliva. Digital transverse microradiography (TMR-D) was used to analyze lesions. Lesion color was monitored spectrophotometrically. The effects of lesion protocol and treatment on changes in lesion depth (ΔLD), mineral loss (ΔΔZ), maximum mineral density at the surface zone (ΔSZmax), and color changes related to remineralization (ΔL*remin) were analyzed using two-way ANOVA.
RESULTS: The treatment×lesion protocol interaction was significant for ΔΔZ (p < 0.01) and ΔL*remin (p < 0.01), however not for ΔLD (p = 0.23) or ΔSZmax (p = 0.91). There were no differences in ΔΔZ between treatments in HEC and C907 lesions. However, DIW resulted in more remineralization than both SDF (p < 0.01) and FV (p = 0.01) in MeC lesions. Considering changes from lesion baseline after remineralization in MeC lesions, SDF treatment resulted in the highest mineral gain in the surface zone. However, DIW revealed the highest mineral gain after remineralization in the lesion body. SDF stained lesions with the intensity increasing after remineralization in C907 lesions, whereas staining decreased in MeC and HEC lesions.
CONCLUSIONS: High fluoride treatments can interfere with continuous remineralization of caries lesions due to partial arrest. Baseline lesion mineral distribution affects SDF\'s ability to enhance remineralization and the staining caused by SDF.
CONCLUSIONS: SDF is being used to arrest active caries lesions extending into dentin and to treat dentin hypersensitivity. This study shed light on SDF\'s effect on an isolated process in dental caries only, remineralization. It achieved this by examining enamel caries lesions with differing mineral distributions and assessing their staining properties.
METHODS: This laboratory study followed a 3 [treatment: SDF/fluoride varnish (FV)/deionized water (DIW)] ×3 [lesion protocol: methylcellulose (MeC)/hydroxyethylcellulose (HEC)/Carbopol 907 (C907)] factorial design. Lesions were created in bovine enamel specimens (n = 20). Treatments were applied and lesions remineralized in artificial saliva. Digital transverse microradiography (TMR-D) was used to analyze lesions. Lesion color was monitored spectrophotometrically. The effects of lesion protocol and treatment on changes in lesion depth (ΔLD), mineral loss (ΔΔZ), maximum mineral density at the surface zone (ΔSZmax), and color changes related to remineralization (ΔL*remin) were analyzed using two-way ANOVA.
RESULTS: The treatment×lesion protocol interaction was significant for ΔΔZ (p < 0.01) and ΔL*remin (p < 0.01), however not for ΔLD (p = 0.23) or ΔSZmax (p = 0.91). There were no differences in ΔΔZ between treatments in HEC and C907 lesions. However, DIW resulted in more remineralization than both SDF (p < 0.01) and FV (p = 0.01) in MeC lesions. Considering changes from lesion baseline after remineralization in MeC lesions, SDF treatment resulted in the highest mineral gain in the surface zone. However, DIW revealed the highest mineral gain after remineralization in the lesion body. SDF stained lesions with the intensity increasing after remineralization in C907 lesions, whereas staining decreased in MeC and HEC lesions.
CONCLUSIONS: High fluoride treatments can interfere with continuous remineralization of caries lesions due to partial arrest. Baseline lesion mineral distribution affects SDF\'s ability to enhance remineralization and the staining caused by SDF.
CONCLUSIONS: SDF is being used to arrest active caries lesions extending into dentin and to treat dentin hypersensitivity. This study shed light on SDF\'s effect on an isolated process in dental caries only, remineralization. It achieved this by examining enamel caries lesions with differing mineral distributions and assessing their staining properties.
摘要：
目的：研究基线矿物质分布是否调节氟化银二胺（SDF）再矿化和染色釉质龋病变的能力。
方法:本实验室研究遵循3[处理:SDF/氟化物清漆(FV)/去离子水(DIW)]×3[损伤方案:甲基纤维素(MeC)/羟乙基纤维素(HEC)/Carbopol907(C907)]析因设计。在牛牙釉质标本中产生损伤(n=20)。应用治疗并在人工唾液中再矿化病变。使用数字横向显微放射成像（TMR-D）分析病变。用分光光度法监测病变颜色。病变方案和治疗对病变深度（ΔLD）变化的影响，矿物损失(ΔΔZ)，地表区域的最大矿物密度(ΔSZmax)，使用双向ANOVA分析与再矿化相关的颜色变化(ΔL*remin)。
结果：治疗×病变方案的相互作用对于ΔΔZ（p<0.01）和ΔL*remin（p<0.01）是显着的，然而对于ΔLD(p=0.23)或ΔSZmax(p=0.91)则不是。HEC和C907病变的治疗之间的ΔΔZ没有差异。然而,在MeC病变中，DIW比SDF（p<0.01）和FV（p=0.01）导致更多的再矿化。考虑到MeC病变再矿化后病变基线的变化，SDF处理在地表区域产生最高的矿物增益。然而,DIW显示病变体中再矿化后矿物质增加最高。SDF染色的C907病变再矿化后强度增加，而MeC和HEC病变的染色减少。
结论：高氟治疗可以干扰由于部分停滞导致的龋齿病变的持续再矿化。基线病变矿物质分布影响SDF增强再矿化的能力和SDF引起的染色。
结论：SDF被用于阻止延伸至牙本质的活动性龋齿病变和治疗牙本质超敏反应。这项研究仅阐明了SDF对龋齿中孤立过程的影响，再矿化。通过检查具有不同矿物质分布的釉质龋齿病变并评估其染色特性来实现这一目标。
方法:本实验室研究遵循3[处理:SDF/氟化物清漆(FV)/去离子水(DIW)]×3[损伤方案:甲基纤维素(MeC)/羟乙基纤维素(HEC)/Carbopol907(C907)]析因设计。在牛牙釉质标本中产生损伤(n=20)。应用治疗并在人工唾液中再矿化病变。使用数字横向显微放射成像（TMR-D）分析病变。用分光光度法监测病变颜色。病变方案和治疗对病变深度（ΔLD）变化的影响，矿物损失(ΔΔZ)，地表区域的最大矿物密度(ΔSZmax)，使用双向ANOVA分析与再矿化相关的颜色变化(ΔL*remin)。
结果：治疗×病变方案的相互作用对于ΔΔZ（p<0.01）和ΔL*remin（p<0.01）是显着的，然而对于ΔLD(p=0.23)或ΔSZmax(p=0.91)则不是。HEC和C907病变的治疗之间的ΔΔZ没有差异。然而,在MeC病变中，DIW比SDF（p<0.01）和FV（p=0.01）导致更多的再矿化。考虑到MeC病变再矿化后病变基线的变化，SDF处理在地表区域产生最高的矿物增益。然而,DIW显示病变体中再矿化后矿物质增加最高。SDF染色的C907病变再矿化后强度增加，而MeC和HEC病变的染色减少。
结论：高氟治疗可以干扰由于部分停滞导致的龋齿病变的持续再矿化。基线病变矿物质分布影响SDF增强再矿化的能力和SDF引起的染色。
结论：SDF被用于阻止延伸至牙本质的活动性龋齿病变和治疗牙本质超敏反应。这项研究仅阐明了SDF对龋齿中孤立过程的影响，再矿化。通过检查具有不同矿物质分布的釉质龋齿病变并评估其染色特性来实现这一目标。
