Abstract：
Hematopoietic stem cells (HSCs) possess the ability to sustain the continuous production of all blood cell types throughout an organism\'s lifespan. Although primarily located in the bone marrow of adults, HSCs originate during embryonic development. Visualization of the birth of HSCs, their developmental trajectory, and the specific interactions with their successive niches have significantly contributed to our understanding of the biology and mechanics governing HSC formation and expansion. Intravital techniques applied to live embryos or non-fixed samples have remarkably provided invaluable insights into the cellular and anatomical origins of HSCs. These imaging technologies have also shed light on the dynamic interactions between HSCs and neighboring cell types within the surrounding microenvironment or niche, such as endothelial cells or macrophages. This review delves into the advancements made in understanding the origin, production, and cellular interactions of HSCs, particularly during the embryonic development of mice and zebrafish, focusing on studies employing (live) imaging analysis.
摘要：
造血干细胞（HSC）具有在生物体的整个生命周期中维持所有血细胞类型的连续生产的能力。虽然主要位于成年人的骨髓中，HSC起源于胚胎发育。HSC出生的可视化，他们的发展轨迹，与它们连续的壁龛的特定相互作用极大地促进了我们对控制HSC形成和扩展的生物学和力学的理解。应用于活胚胎或非固定样品的体内技术已经显著提供了对HSC的细胞和解剖起源的宝贵见解。这些成像技术还揭示了HSC与周围微环境或小生境中相邻细胞类型之间的动态相互作用。如内皮细胞或巨噬细胞。这篇综述深入探讨了在理解起源方面取得的进步，生产,和HSC的细胞相互作用，特别是在小鼠和斑马鱼的胚胎发育过程中，专注于采用(实时)成像分析的研究。
