Abstract：
As one of endogenous reactive oxygen species (ROS), peroxynitrite (ONOO-) performs various functions in both pathological and physiological mechanisms. In this work, an optical and near-infrared (NIR) fluorescent probe (NX), which based on 3-dihydro-1H-xanthene and 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) group was designed and prepared to detect ONOO-. This probe revealed an obvious optical and a fluorescent response when ONOO- was present and it exhibited higher selectivity over other ROS. Especially, the dual NIR fluorescence changes at 660 and 800 nm allowed quantitative detection of ONOO- in the range of 15-40 μM, and the detection limit was 82 nM. Finally, the probe was effectively employed to visualize exogenous and endogenous ONOO- in HepG2 cells and zebrafish, respectively. All the results indicated the dual NIR-channel probe could serve as a potent detecting tools in studying ONOO- in vitro and in vivo.
摘要：
作为内源性活性氧（ROS）之一，过氧亚硝酸盐（ONOO-）在病理和生理机制中执行各种功能。在这项工作中,光学和近红外（NIR）荧光探针（NX），设计并制备了基于3-二氢-1H-氧杂蒽和2-二氰基-3-氰基-4,5-三甲基-2,5-二氢呋喃（TCF）基团的ONOO-检测方法。当ONOO-存在时，该探针显示出明显的光学和荧光响应，并且相对于其他ROS表现出更高的选择性。尤其是,在660和800nm的双近红外荧光变化允许ONOO-在15-40μM的范围内定量检测，检测极限为82nM。最后,该探针有效地用于可视化HepG2细胞和斑马鱼中的外源性和内源性ONOO-，分别。所有结果表明，双NIR通道探针可以作为体外和体内研究ONOO-的有效检测工具。
