Abstract：
The proliferation and differentiation of skeletal muscle satellite cells is a complex physiological process involving various transcription factors and small RNA molecules. This study aimed to understand the regulatory mechanisms underlying these processes, focusing on interferon-related development factor 2 (IFRD2) as a target gene of miRNA-2400 in bovine skeletal MuSCs (MuSCs). IFRD2 was identified as a target gene of miRNA-2400 involved in regulating the proliferation and differentiation of bovine skeletal MuSCs. Our results indicate that miR-2400 can target binding the 3\'UTR of IFRD2 and inhibit its translation. mRNA and protein expression levels of IFRD2 increased significantly with increasing days of differentiation. Moreover, overexpression of the IFRD2 gene inhibited proliferation and promoted differentiation of bovine MuSCs. Conversely, the knockdown of the gene had the opposite effect. Overexpression of IFRD2 resulted in the inhibition of ERK1/2 phosphorylation levels in bovine MuSCs, which in turn promoted differentiation. In summary, IFRD2, as a target gene of miR-2400, crucially affects bovine skeletal muscle proliferation and differentiation by precisely regulating ERK1/2 phosphorylation.
摘要：
骨骼肌卫星细胞的增殖和分化是一个复杂的生理过程，涉及多种转录因子和小RNA分子。本研究旨在了解这些过程背后的调节机制，研究了干扰素相关发展因子2(IFRD2)作为牛骨骼MuSCs(MuSCs)miRNA-2400的靶基因。IFRD2被鉴定为miRNA-2400的靶基因，参与调节牛骨骼MuSCs的增殖和分化。我们的结果表明miR-2400可以靶向结合IFRD2的3'UTR并抑制其翻译。随着分化天数的增加，IFRD2的mRNA和蛋白表达水平显着增加。此外，IFRD2基因的过表达抑制了牛MuSC的增殖并促进了分化。相反，基因的敲除产生了相反的效果。IFRD2的过表达导致牛MuSCs中ERK1/2磷酸化水平的抑制,这反过来又促进了分化。总之,IFRD2作为miR-2400的靶基因,通过精确调控ERK1/2磷酸化对牛骨骼肌的增殖和分化产生重要影响。
