Abstract：
The removal of double-stranded RNA (dsRNA) contaminants during in vitro mRNA synthesis is one of the technological problems to be solved. Apparently, these contaminants are the result of the T7 RNA polymerase side activity. In this study, we used a modified method of mRNA purification based on the selective binding of dsRNA to cellulose in ethanol-containing buffer. It was shown both in vivo and in vitro that the cellulose-purified mRNA preparation leads neither to activation of the lymphocyte inflammatory marker CD69 nor to increased release of IFNα in mice, and does not contain impurities detectable by antibodies to dsRNA.
摘要：
在体外mRNA合成过程中去除双链RNA（dsRNA）污染物是需要解决的技术问题之一。显然,这些污染物是T7RNA聚合酶副活性的结果。在这项研究中,我们使用了一种改良的mRNA纯化方法,该方法基于dsRNA在含乙醇的缓冲液中与纤维素的选择性结合。在体内和体外都表明，纤维素纯化的mRNA制剂既不会导致淋巴细胞炎症标志物CD69的激活，也不会导致小鼠中IFNα的释放增加。并且不含有可通过dsRNA抗体检测的杂质。
