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Abstract：
Bacteriocins have the potential to effectively improve food-borne infections or gastrointestinal diseases and hold promise as viable alternatives to antibiotics. This study aimed to explore the antibacterial activity of three bacteriocins (nisin, enterocin Gr17, and plantaricin RX-8) and their ability to attenuate intestinal barrier dysfunction and inflammatory responses induced by Listeria monocytogenes, respectively. Bacteriocins have shown excellent antibacterial activity against L. monocytogenes without causing any cytotoxicity. Bacteriocins inhibited the adhesion and invasion of L. monocytogenes on Caco-2 cells, lactate dehydrogenase (LDH), trans-epithelial electrical resistance (TEER), and cell migration showed that bacteriocin improved the permeability of Caco-2 cells. These results were attributed to the promotion of tight junction proteins (TJP) assembly, specifically zonula occludens-1 (ZO-1), occludin, and claudin-1. Furthermore, bacteriocins could alleviate inflammation by inhibiting the mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) pathways and reducing the secretion of interleukin-6 (IL-6), interleukin-1 β (IL-1β) and tumor necrosis factor α (TNF-α). Among three bacteriocins, plantaricin RX-8 showed the best antibacterial activity against L. monocytogenes and the most pronounced protective effect on the intestinal barrier due to its unique structure. Based on our findings, we hypothesized that bacteriocins may inhibit the adhesion and invasion of L. monocytogenes by competing adhesion sites. Moreover, they may further enhance intestinal barrier function by inhibiting the expression of L. monocytogenes virulence factors, increasing the expression of TJP and decreasing the secretion of inflammatory factors. Therefore, bacteriocins will hopefully be an effective alternative to antibiotics, and this study provides valuable insights into food safety concerns. KEY POINTS: • Bacteriocins show excellent antibacterial activity against L. monocytogenes • Bacteriocins improve intestinal barrier damage and inflammatory response • Plantaricin RX-8 has the best protective effect on Caco-2 cells damage.
摘要：
细菌素有可能有效改善食源性感染或胃肠道疾病，并有望成为抗生素的可行替代品。本研究旨在探讨三种细菌素（Nisin，肠霉素Gr17和植物乳杆菌素RX-8）及其减弱单核细胞增生李斯特菌诱导的肠屏障功能障碍和炎症反应的能力，分别。细菌素对单核细胞增生李斯特菌显示出优异的抗菌活性，而不会引起任何细胞毒性。细菌素抑制单核细胞增生李斯特菌对Caco-2细胞的粘附和侵袭,乳酸脱氢酶（LDH），跨上皮电阻（TEER），和细胞迁移表明细菌素改善了Caco-2细胞的通透性。这些结果归因于紧密连接蛋白（TJP）组装的促进，特别是小带闭塞-1(ZO-1)，occludin,还有Claudin-1.此外，细菌素可通过抑制丝裂原活化蛋白激酶(MAPK)和核因子κB(NF-κB)通路,减少白细胞介素-6(IL-6)的分泌,减轻炎症,白细胞介素-1β（IL-1β）和肿瘤坏死因子α（TNF-α）。在三种细菌素中，植物乳杆菌素RX-8由于其独特的结构，对单核细胞增生李斯特菌具有最佳的抗菌活性，对肠屏障具有最明显的保护作用。根据我们的发现,我们假设细菌素可能通过竞争性粘附位点抑制单核细胞增生李斯特菌的粘附和侵袭。此外，它们可能通过抑制单核细胞增生李斯特菌毒力因子的表达进一步增强肠道屏障功能，增加TJP的表达和减少炎症因子的分泌。因此,细菌素有望成为抗生素的有效替代品，这项研究为食品安全问题提供了有价值的见解。关键点：•细菌素对单核细胞增生李斯特菌表现出优异的抗菌活性•细菌素改善肠道屏障损伤和炎症反应•植物乳杆菌素RX-8对Caco-2细胞损伤具有最佳保护作用。
