PDF(Pubmed)
Abstract：
In this report, we show that a very common modification (especially in tRNA), dihydrouridine, was efficiently produced by photoreduction of the canonical pyrimidine ribonucleoside, uridine in formamide. Formamide not only acts as a solvent in this reaction, but also as the reductant. The other three components of the canonical alphabet (C, A, G) remained intact under the same conditions, suggesting that dihydrouridine might have coexisted with all four canonical RNA nucleosides (C, U, A, G) at the dawn of life.
摘要：
在这份报告中,我们证明了一种非常常见的修饰（尤其是在tRNA中），二氢尿苷,通过经典嘧啶核糖核苷的光还原有效产生，尿苷在甲酰胺中。甲酰胺不仅在该反应中充当溶剂，也可以作为还原剂。规范字母的其他三个组成部分（C，A,G)在相同条件下保持完好无损，表明二氢尿苷可能与所有四个经典RNA核苷共存(C，U,A,G）在生命的黎明。
