PDF(Pubmed)
Abstract：
The mangrove fish (Oryzias curvinotus) serves as a model for researching environmental adaptation and sexual development. To further such research, we sequenced and assembled a high-quality 842 Mb reference genome for O. curvinotus. Comparative genomic analysis revealed 891 expanded gene families, including significantly expanded cytochrome P450 (CYP) detoxification genes known to be involved in xenobiotic defense. We identified 69 O. curvinotus CYPs (OcuCYPs) across 18 families and 10 clans using multiple methods. Extensive RNA-seq and qPCR analysis demonstrated diverse spatiotemporal expression patterns of OcuCYPs by developmental stage, tissue type, sex, and pollutant exposure (17β-estradiol (E2) and testosterone (MT)). Many OcuCYPs exhibited sexual dimorphism in gonads, suggesting reproductive roles in steroidogenesis, while their responsiveness to model toxicants indicates their importance in environmental adaptation through enhanced detoxification. Pathway analysis highlighted expanded CYP genes in arachidonic acid metabolism, drug metabolism, and steroid hormone biosynthesis. This chromosome-level genomic resource provides crucial biological insights to elucidate the functional roles of expanded CYPs in environmental adaptation, sexual development, early life history, and conservation in the anthropogenically impacted mangrove habitats of O. curvinotus. It also enables future ecotoxicology research leveraging O. curvinotus as a pollution sentinel species.
摘要：
红树林鱼（Oryziascurvinotus）是研究环境适应和性发育的模型。为了进一步研究，我们对O.curvinotus的高质量842Mb参考基因组进行了测序和组装。比较基因组分析显示891个扩展的基因家族，包括已知与异种生物防御有关的显着扩增的细胞色素P450（CYP）解毒基因。我们使用多种方法鉴定了18个家族和10个氏族的69个O.curvinotusCYPs（OcuCYPs）。广泛的RNA-seq和qPCR分析证明了OcuCYPs在发育阶段的不同时空表达模式，组织类型,性别,和污染物暴露(17β-雌二醇(E2)和睾酮(MT))。许多OcuCYPs在性腺中表现出性二态，提示在类固醇生成中的生殖作用，而它们对模型毒物的反应表明了它们通过增强解毒在环境适应中的重要性。路径分析强调了花生四烯酸代谢中扩增的CYP基因，药物代谢，和类固醇激素的生物合成。这种染色体水平的基因组资源提供了关键的生物学见解，以阐明扩展的CYP在环境适应中的功能作用。性发育,早期生活史,以及受人为影响的O.curvinotus红树林栖息地的保护。它还可以利用O.curvinotus作为污染哨兵物种进行未来的生态毒理学研究。
