PDF(Pubmed)
Abstract：
UNASSIGNED: Black spots (BSs) are lentiginous findings observed in the gastric body and fundus during upper gastrointestinal endoscopy and are predominantly seen in patients undergoing Helicobacter pylori eradication treatment. However, the detailed patient background and exact composition are poorly understood. This study aims to clarify the clinicopathological features of BSs, examine patient demographics, and use the NanoSuit-correlative light and electron microscopy (CLEM) method combined with scanning electron microscopy-energy dispersive X-ray spectroscopy for elemental analysis.
UNASSIGNED: Patients who underwent upper gastrointestinal endoscopy between 2017 and 2022 were included. Data on age, medications, blood tests, and H. pylori infection status were retrospectively gathered from medical records. Univariate analysis was conducted to examine BS presence, with results then used in a multivariate model to identify associated risk factors. Additionally, pathological specimens from patients with BSs were analyzed for elemental composition using the NanoSuit-CLEM method combined with scanning electronmicroscopy-energy dispersive X-ray spectroscopy.
UNASSIGNED: An analysis of 6778 cases identified risk factors for BSs, including older age and using proton pump inhibitors, statins, corticosteroids, and antithrombotic drugs. Endoscopically, BSs correlated with higher gastric atrophy and lower active H. pylori infection. Iron deposition at BS sites was specifically identified using NanoSuit-CLEM.
UNASSIGNED: BSs on gastrointestinal endoscopy may indicate an absence of active H. pylori inflammation. The discovery of iron deposition within BSs using the NanoSuit-CLEM method has offered new insights into the possible causative factors and advances our understanding of the etiology of BSs, bringing us closer to unraveling the underlying mechanisms of their formation.
UNASSIGNED: Patients who underwent upper gastrointestinal endoscopy between 2017 and 2022 were included. Data on age, medications, blood tests, and H. pylori infection status were retrospectively gathered from medical records. Univariate analysis was conducted to examine BS presence, with results then used in a multivariate model to identify associated risk factors. Additionally, pathological specimens from patients with BSs were analyzed for elemental composition using the NanoSuit-CLEM method combined with scanning electronmicroscopy-energy dispersive X-ray spectroscopy.
UNASSIGNED: An analysis of 6778 cases identified risk factors for BSs, including older age and using proton pump inhibitors, statins, corticosteroids, and antithrombotic drugs. Endoscopically, BSs correlated with higher gastric atrophy and lower active H. pylori infection. Iron deposition at BS sites was specifically identified using NanoSuit-CLEM.
UNASSIGNED: BSs on gastrointestinal endoscopy may indicate an absence of active H. pylori inflammation. The discovery of iron deposition within BSs using the NanoSuit-CLEM method has offered new insights into the possible causative factors and advances our understanding of the etiology of BSs, bringing us closer to unraveling the underlying mechanisms of their formation.
摘要：
■黑点（BS）是上消化道内镜检查期间在胃体和胃底观察到的轻微发现，主要见于接受幽门螺杆菌根除治疗的患者。然而,详细的患者背景和确切的成分知之甚少。本研究旨在阐明BS的临床病理特征，检查患者的人口统计学，并使用NanoSuit相关光学和电子显微镜（CLEM）方法结合扫描电子显微镜-能量色散X射线光谱法进行元素分析。
■纳入了2017年至2022年间接受上消化道内镜检查的患者。年龄数据,药物,验血,和H.pylori感染状况从病历中回顾性收集.进行单变量分析以检查BS的存在，然后将结果用于多变量模型中以识别相关的风险因素。此外,使用NanoSuit-CLEM方法结合扫描电子显微镜-能量色散X射线光谱法分析来自BS患者的病理标本的元素组成。
■对6778例病例的分析确定了BS的风险因素，包括年龄较大和使用质子泵抑制剂，他汀类药物，皮质类固醇,和抗血栓药物.内窥镜检查,BSs与较高的胃萎缩和较低的活动性幽门螺杆菌感染相关。使用NanoSuit-CLEM特别鉴定了BS位点处的铁沉积。
■胃肠内窥镜检查的BS可能表明没有活动性幽门螺杆菌炎症。使用NanoSuit-CLEM方法发现BS中的铁沉积为可能的致病因素提供了新的见解，并促进了我们对BS病因的理解。让我们更接近解开它们形成的潜在机制。
■纳入了2017年至2022年间接受上消化道内镜检查的患者。年龄数据,药物,验血,和H.pylori感染状况从病历中回顾性收集.进行单变量分析以检查BS的存在，然后将结果用于多变量模型中以识别相关的风险因素。此外,使用NanoSuit-CLEM方法结合扫描电子显微镜-能量色散X射线光谱法分析来自BS患者的病理标本的元素组成。
■对6778例病例的分析确定了BS的风险因素，包括年龄较大和使用质子泵抑制剂，他汀类药物，皮质类固醇,和抗血栓药物.内窥镜检查,BSs与较高的胃萎缩和较低的活动性幽门螺杆菌感染相关。使用NanoSuit-CLEM特别鉴定了BS位点处的铁沉积。
■胃肠内窥镜检查的BS可能表明没有活动性幽门螺杆菌炎症。使用NanoSuit-CLEM方法发现BS中的铁沉积为可能的致病因素提供了新的见解，并促进了我们对BS病因的理解。让我们更接近解开它们形成的潜在机制。
