PDF(Pubmed)
Abstract：
Tyrosinase is one important rate limiting enzyme in melanin synthesis, directly affecting the melanin synthesis. Quercetagetin is one active ingredient from marigold. Thence, the inhibition effects of quercetagetin against tyrosinase were investigated. The results showed quercetagetin could inhibit tyrosinase activity with IC50 value of 0.19 ± 0.01 mM and the inhibition type was a reversible mixed-type. Results of fluorescence quenching showed quercetagetin could quench tyrosinase fluorescence in static process. CD and 3D fluorescence results showed the interaction of quercetagetin to tyrosinase could change tyrosinase conformation to inhibit activity. Moreover, docking revealed details of quercetagetin\'s interactions with tyrosinase.
摘要：
酪氨酸酶是黑色素合成中重要的限速酶,直接影响黑色素的合成。槲皮素是万寿菊的一种活性成分。从那里，研究槲皮素对酪氨酸酶的抑制作用。结果表明，槲皮素能抑制酪氨酸酶活性，IC50为0.19±0.01mM，抑制类型为可逆混合型。荧光猝灭成果显示槲皮素能在静态进程中猝灭酪氨酸酶的荧光。CD和3D荧光结果表明槲皮素与酪氨酸酶的相互作用可以改变酪氨酸酶的构象以抑制活性。此外，对接显示槲皮素与酪氨酸酶相互作用的细节。
