PDF(Pubmed)
Abstract：
Ammonium polyphosphate (APP), a pivotal constituent within environmentally friendly flame retardants, exhibits notable decomposition susceptibility and potentially engenders ecological peril. Consequently, monitoring the APP concentration to ensure product integrity and facilitate the efficacious management of wastewater from production processes is of great significance. A fluorescent assay was devised to swiftly discern APP utilizing 4\',6\'-diamino-2-phenylindole (DAPI). With increasing APP concentrations, DAPI undergoes intercalation within its structure, emitting pronounced fluorescence. Notably, the flame retardant JLS-PNA220-A, predominantly comprising APP, was employed as the test substrate. Establishing a linear relationship between fluorescence intensity (F-F0) and JLS-PNA220-A concentration yielded the equation y = 76.08x + 463.2 (R2 = 0.9992), with a LOD determined to be 0.853 mg/L. The method was used to assess the degradation capacity of APP-degrading bacteria. Strain D-3 was isolated, and subsequent analysis of its 16S DNA sequence classified it as belonging to the Acinetobacter genus. Acinetobacter nosocomialis D-3 demonstrated superior APP degradation capabilities under pH 7 at 37 °C, with degradation rates exceeding 85% over a four-day cultivation period. It underscores the sensitivity and efficacy of the proposed method for APP detection. Furthermore, Acinetobacter nosocomialis D-3 exhibits promising potential for remediation of residual APP through environmental biodegradation processes.
摘要：
聚磷酸铵(APP),环保阻燃剂中的关键成分，表现出明显的分解敏感性，并可能造成生态危险。因此,监测APP浓度对确保产品完整性和促进生产过程废水的有效管理具有重要意义。设计了一种荧光测定法，利用4'快速辨别APP，6'-二氨基-2-苯基吲哚(DAPI)。随着APP浓度的增加，DAPI在其结构中经历嵌入，发射明显的荧光。值得注意的是,阻燃剂JLS-PNA220-A,主要包括APP，用作测试基底。建立荧光强度（F-F0）与JLS-PNA220-A浓度之间的线性关系，得出方程y=76.08x463.2（R2=0.9992），LOD确定为0.853mg/L该方法用于评估APP降解菌的降解能力。分离菌株D-3，随后对其16SDNA序列的分析将其归类为不动杆菌属。不动杆菌D-3在37°C的pH7下表现出优异的APP降解能力，在四天的栽培期内降解率超过85%。它强调了所提出的APP检测方法的灵敏度和功效。此外，不动杆菌D-3具有通过环境生物降解过程修复残留APP的潜力。
