Abstract:
Herbal medicine has been widely valued because of its remarkable efficacy and minimal side effects. The quantitative analysis of herbal medicines is essential to ensure their safety and efficacy. The simultaneous detection of multiple quality markers (Q-markers) has emerged as an important approach and trend in herbal medicine quality control. In recent years, non-targeted screening has become an effective strategy for the discovery and identification of unknown compounds. This study developed a non-targeted screening and quantitative analysis strategy to discover, identify and quantify the multiple components that truly represent the efficacy of Wuling capsule. Within this strategy, 18 types of flavonoids were tentatively discovered and identified from Wuling capsule by analyzing mass cleavage pathways, the precise molecular weights of compounds, and comparing the data with a database. Ten types of flavonoids were determined after the comparison of the standards. Additionally, following the evaluation of the regression equation, linear range, limit of detection (LOD), limit of quantitation (LOQ), precision, repeatability, and recovery of the proposed quantitative method, six flavonoids were quantified. This method successfully screened, identified, and quantified the potential active components in Wuling capsule, providing insights for improving the quality control standards in other herbal medicines.
摘要:
中草药因其疗效显著、副作用小而受到广泛的重视。草药的定量分析对于确保其安全性和有效性至关重要。同时检测多种质量标记(Q标记)已成为草药质量控制的重要方法和趋势。近年来,非靶向筛查已成为发现和鉴定未知化合物的有效策略.这项研究开发了一种非针对性的筛查和定量分析策略,以发现,识别和量化真正代表五灵胶囊功效的多种成分。在这一战略中,通过分析质量裂解途径,从五灵胶囊中初步发现并鉴定了18种黄酮类化合物,化合物的精确分子量,并将数据与数据库进行比较。比较标准后确定了十种类黄酮。此外,在回归方程的评估之后,线性范围,检测限(LOD),定量限(LOQ),精度,重复性,并恢复了所提出的定量方法,对六种类黄酮进行了定量。该方法成功筛选,已识别,并量化了五灵胶囊中潜在的活性成分,为提高其他草药的质量控制标准提供见解。