Abstract:
The pomegranate processing industry generates worldwide enormous amounts of by-products, such as pomegranate peels (PPs), which constitute a rich source of phenolic compounds. In this view, PPs could be exploited as a sustainable source of ellagic acid, which is a compound that possesses various biological actions. The present study aimed at the liberation of ellagic acid from its bound forms via ultrasound-assisted alkaline hydrolysis, which was optimized using response surface methodology. The effects of duration of sonication, solvent:solid ratio, and NaOH concentration on total phenol content (TPC), antioxidant activity, and punicalagin and ellagic acid content were investigated. Using the optimum hydrolysis conditions (i.e., 32 min, 1:48 v/w, 1.5 mol/L NaOH), the experimental responses were found to be TCP: 4230 ± 190 mg GAE/100 g dry PPs; AABTS: 32,398 ± 1817 µmol Trolox/100 g dry PPs; ACUPRAC: 29,816 ± 1955 µmol Trolox/100 g dry PPs; 59 ± 3 mg punicalagin/100 g dry PPs; and 1457 ± 71 mg ellagic acid/100 g dry PPs. LC-QTOF-MS and GC-MS analysis of the obtained PP extract revealed the presence of various phenolic compounds (e.g., ellagic acid), organic acids (e.g., citric acid), sugars (e.g., fructose) and amino acids (e.g., glycine). The proposed methodology could be of use for food, pharmaceutical, and cosmetics applications, thus reinforcing local economies.
摘要:
石榴加工业在全球范围内产生了大量的副产品,如石榴皮(PPs),构成酚类化合物的丰富来源。在这个观点中,PPs可以用作鞣花酸的可持续来源,这是一种具有各种生物作用的化合物。本研究旨在通过超声辅助碱性水解从其结合形式中释放鞣花酸,采用响应面法进行了优化。超声处理持续时间的影响,溶剂:固体比,和NaOH浓度对总酚含量(TPC),抗氧化活性,并对石榴苷和鞣花酸含量进行了研究。使用最佳水解条件(即,32分钟,1:48v/w,1.5mol/LNaOH),实验结果为TCP:4230±190mgGAE/100g干PPs;AABTS:32,398±1817µmolTrolox/100g干PPs;ACUPRAC:29,816±1955µmolTrolox/100g干PPs;59±3mgpunicalagin/100g干PPs;1457±71mg鞣花酸/100g干PPs.获得的PP提取物的LC-QTOF-MS和GC-MS分析显示存在各种酚类化合物(例如,鞣花酸),有机酸(例如,柠檬酸),糖(例如,果糖)和氨基酸(例如,甘氨酸)。拟议的方法可能对食物有用,Pharmaceutical,和化妆品应用,从而加强当地经济。