PDF(Pubmed)
Abstract：
Background/Objectives: The aim of this study was to establish a histologic baseline for cryoanalgesia of 2 min duration and evaluate the effects of different freeze durations. Methods: A porcine model was used in which the application of bilateral cryoanalgesia from intercostal spaces T3-T7 was completed via partial median sternotomy. The animals were kept alive for 7 days and the ribcages were sent to a specialized center for histopathologic analysis of the freezing injury. Results: Forty freezing lesions were completed and analyzed histologically. Thirty-eight (95%) of the cryo-lesions presented 100% nerve fiber degeneration at or distal to the ablation site, with preservation of the perineural connective tissue, as intended. The two unaffected nerves were found to be physically located outside of the freezing area. Conclusions: The complete axonal degeneration with preservation of the perineural tissue opens the possibility to shorter freezing times than the recommended 2 min. Visualization of the nerve and positioning of the probe is important in ensuring the proper effect on the nerve. This histologic analysis confirms the process triggered by cryoanalgesia that, until now, had only been assumed.
摘要：
背景/目的：本研究的目的是建立2分钟冷冻镇痛的组织学基线，并评估不同冷冻持续时间的效果。方法：采用猪模型，通过部分正中胸骨切开术完成肋间间隙T3-T7双侧冷冻镇痛的应用。将动物存活7天，并将胸肌送到专门的中心进行冷冻损伤的组织病理学分析。结果：共完成40个冰冻病灶并进行组织学分析。38个（95%）冷冻损伤在消融部位或远端表现出100%的神经纤维变性，保留神经周结缔组织，如意。发现两个未受影响的神经物理上位于冷冻区域之外。结论：保留神经周组织的完整轴突变性使冷冻时间比推荐的2分钟短。神经的可视化和探针的定位对于确保对神经的适当影响是重要的。这项组织学分析证实了冷冻镇痛引发的过程，直到现在,只是被假定了。
