PDF(Pubmed)
Abstract：
Despite substantial evidence supporting the efficacy of prebiotics for promoting host health and stress resilience, few experiments present evidence documenting the dynamic changes in microbial ecology and fecal microbially modified metabolites over time. Furthermore, the literature reports a lack of reproducible effects of prebiotics on specific bacteria and bacterial-modified metabolites. The current experiments examined whether consumption of diets enriched in prebiotics (galactooligosaccharides (GOS) and polydextrose (PDX)), compared to a control diet, would consistently impact the gut microbiome and microbially modified bile acids over time and between two research sites. Male Sprague Dawley rats were fed control or prebiotic diets for several weeks, and their gut microbiomes and metabolomes were examined using 16S rRNA gene sequencing and untargeted LC-MS/MS analysis. Dietary prebiotics altered the beta diversity, relative abundance of bacterial genera, and microbially modified bile acids over time. PICRUSt2 analyses identified four inferred functional metabolic pathways modified by the prebiotic diet. Correlational network analyses between inferred metabolic pathways and microbially modified bile acids revealed deoxycholic acid as a potential network hub. All these reported effects were consistent between the two research sites, supporting the conclusion that dietary prebiotics robustly changed the gut microbial ecosystem. Consistent with our previous work demonstrating that GOS/PDX reduces the negative impacts of stressor exposure, we propose that ingesting a diet enriched in prebiotics facilitates the development of a health-promoting gut microbial ecosystem.
摘要：
尽管有大量证据支持益生元促进宿主健康和压力恢复能力的功效，很少有实验证明微生物生态学和粪便微生物修饰代谢产物随时间的动态变化。此外，文献报道,益生元对特定细菌和细菌修饰代谢产物缺乏可重复的作用.当前的实验检查了是否食用富含益生元（低聚半乳糖（GOS）和聚葡萄糖（PDX））的饮食，与对照饮食相比，会持续影响肠道微生物组和微生物修饰的胆汁酸随着时间的推移和两个研究地点之间。雄性SpragueDawley大鼠饲喂对照或益生元饮食数周，使用16SrRNA基因测序和非靶向LC-MS/MS分析检查了它们的肠道微生物组和代谢组。膳食益生元改变了β多样性，细菌属的相对丰度，随着时间的推移和微生物修饰的胆汁酸。PICRUSt2分析确定了由益生元饮食修饰的四种推断的功能性代谢途径。推断的代谢途径与微生物修饰的胆汁酸之间的相关网络分析显示，脱氧胆酸是潜在的网络枢纽。所有这些报告的效果在两个研究地点之间是一致的，支持膳食益生元强烈改变肠道微生物生态系统的结论。与我们以前的工作一致，证明GOS/PDX减少了压力源暴露的负面影响，我们建议摄入富含益生元的饮食有助于促进健康的肠道微生物生态系统的发展。
