PDF(Pubmed)
Abstract：
One-carbon metabolism (OCM) is a complex and interconnected network that undergoes drastic changes during pregnancy. In this study, we investigated the longitudinal distribution of OCM-related metabolites in maternal and cord blood and explored their relationships. Additionally, we conducted cross-sectional analyses to examine the interrelationships among these metabolites. This study included 146 healthy pregnant women who participated in the Chiba Study of Mother and Child Health. Maternal blood samples were collected during early pregnancy, late pregnancy, and delivery, along with cord blood samples. We analyzed 18 OCM-related metabolites in serum using stable isotope dilution liquid chromatography/tandem mass spectrometry. We found that serum S-adenosylmethionine (SAM) concentrations in maternal blood remained stable throughout pregnancy. Conversely, S-adenosylhomocysteine (SAH) concentrations increased, and the total homocysteine/total cysteine ratio significantly increased with advancing gestational age. The betaine/dimethylglycine ratio was negatively correlated with total homocysteine in maternal blood for all sampling periods, and this correlation strengthened with advances in gestational age. Most OCM-related metabolites measured in this study showed significant positive correlations between maternal blood at delivery and cord blood. These findings suggest that maternal OCM status may impact fetal development and indicate the need for comprehensive and longitudinal evaluations of OCM during pregnancy.
摘要：
单碳代谢（OCM）是一个复杂且相互连接的网络，在怀孕期间会发生剧烈变化。在这项研究中,我们调查了OCM相关代谢物在母体和脐带血中的纵向分布，并探讨了它们之间的关系。此外,我们进行了横断面分析,以检查这些代谢物之间的相互关系.这项研究包括146名参加千叶母婴健康研究的健康孕妇。孕妇在怀孕早期收集血样，妊娠晚期,和交付，还有脐带血样本.我们使用稳定同位素稀释液相色谱/串联质谱法分析了血清中18种OCM相关代谢物。我们发现母体血液中的血清S-腺苷甲硫氨酸（SAM）浓度在整个怀孕期间保持稳定。相反，S-腺苷同型半胱氨酸（SAH）浓度增加，随着孕龄的增加，总同型半胱氨酸/总半胱氨酸比率显着增加。甜菜碱/二甲基甘氨酸比值与母体血液中的总同型半胱氨酸呈负相关,这种相关性随着胎龄的增加而加强。在这项研究中测量的大多数OCM相关代谢物显示出分娩时的母体血液与脐带血之间的显着正相关。这些发现表明，母体OCM状态可能会影响胎儿发育，并表明需要在怀孕期间对OCM进行全面和纵向评估。
