PDF(Pubmed)
Abstract：
Dendritic cells (DCs) can initiate immune response through the presenting antigens to naïve T lymphocytes. Esculeoside A (EsA), a spirosolane glycoside, is reported as a major component in the ripe fruit of tomato. Little is known about the effect of tomato saponin on mice bone marrow-derived DCs. This study revealed that EsA and its aglycon, esculeogenin A (Esg-A), attenuated the phenotypic and functional maturation of murine DCs stimulated by lipopolysaccharide (LPS). We found that EsA/Esg-A down-regulated the expression of major histocompatibility complex type II molecules and costimulatory molecule CD86 after LPS stimulation. It was also determined that EsA-/Esg-A-treated DCs were poor stimulators of allogeneic T-cell proliferation and exhibited impaired interleukin-12 and TNF-α production. Additionally, EsA/Esg-A was able to inhibit TLR4-related and p-NFκB signaling pathways. This study shows new insights into the immunopharmacology of EsA/Esg-A, and represents a novel approach to controlling DCs for therapeutic application.
摘要：
树突状细胞(DC)可以通过将抗原呈递至初始T淋巴细胞来启动免疫应答。EsculeosideA(EsA),螺溶胶烷糖苷，据报道是番茄成熟果实中的主要成分。关于番茄皂苷对小鼠骨髓来源的DC的影响知之甚少。这项研究表明，EsA及其糖苷，esculeogeninA(Esg-A)，减弱脂多糖（LPS）刺激的鼠DC的表型和功能成熟。我们发现EsA/Esg-A下调LPS刺激后主要组织相容性复合物II型分子和共刺激分子CD86的表达。还确定了EsA-/Esg-A处理的DC是同种异体T细胞增殖的弱刺激物，并表现出白细胞介素12和TNF-α产生受损。此外,EsA/Esg-A能够抑制TLR4相关和p-NFκB信号通路。这项研究显示了对EsA/Esg-A免疫药理学的新见解，并且代表了用于治疗应用的控制DC的新方法。
