PDF(Pubmed)
Abstract：
Yerba Mate (YM) (Ilex paraguariensis) is a natural herbal supplement with a well-described anti-inflammatory capacity and beneficial effects in different inflammatory contexts such as insulin resistance or obesity. However, whether YM could improve other inflammatory conditions such as colitis or the immune cell population that can be modulated by this plant remains elusive. Here, by using 61 male and female C57BL/6/J wild-type (WT) mice and the dextran sodium sulfate (DSS)-induced acute colitis model, we evaluated the effect of YM on colitis symptoms and macrophage polarization. Our results showed that the oral administration of YM reduces colitis symptoms and improves animal survival. Increasing infiltration of anti-inflammatory M2 macrophage was observed in the colon of the mice treated with YM. Accordingly, YM promoted M2 macrophage differentiation in vivo. However, the direct administration of YM to bone marrow-derived macrophages did not increase anti-inflammatory polarization, suggesting that YM, through an indirect mechanism, is able to skew the M1/M2 ratio. Moreover, YM consumption reduced the Eubacterium rectale/Clostridium coccoides and Enterobacteriaceae groups and increased the Lactobacillus/Lactococcus group in the gut microbiota. In summary, we show that YM promotes an immunosuppressive environment by enhancing anti-inflammatory M2 macrophage differentiation, reducing colitis symptoms, and suggesting that YM consumption may be a good cost-effective treatment for ulcerative colitis.
摘要：
YerbaMate（YM）（Ilexparaguariensis）是一种天然的草药补充剂，具有良好的抗炎能力和在胰岛素抵抗或肥胖等不同炎症环境中的有益作用。然而,YM是否可以改善其他炎症状况，例如结肠炎或可以由该植物调节的免疫细胞群体仍然难以捉摸。这里,采用61只雄性和雌性C57BL/6/J野生型(WT)小鼠和葡聚糖硫酸钠(DSS)诱导的急性结肠炎模型,我们评估了YM对结肠炎症状和巨噬细胞极化的影响。我们的结果表明，口服YM可减少结肠炎症状并改善动物存活率。在用YM处理的小鼠的结肠中观察到抗炎M2巨噬细胞的浸润增加。因此，YM在体内促进M2巨噬细胞分化。然而,YM对骨髓源性巨噬细胞的直接给药不会增加抗炎极化，暗示YM,通过间接机制，能够扭曲M1/M2比率。此外，YM的消耗减少了肠杆菌/球菌梭状芽胞杆菌和肠杆菌科群，并增加了肠道微生物群中的乳杆菌/乳球菌群。总之,我们显示YM通过增强抗炎M2巨噬细胞分化促进免疫抑制环境，减少结肠炎症状，并表明YM可能是溃疡性结肠炎的一种经济有效的治疗方法。
