PDF(Pubmed)
Abstract：
Skin problems caused by aging have attracted much attention, and marine collagen peptides have been proved to improve these problems, while mammalian collagen peptides are rarely reported. In this study, fermented deer bone collagen peptide (FCP) and non-fermented deer bone collagen peptide (NCP) were extracted from fermented and non-fermented deer bone, respectively, and their peptide sequences and differential proteins were analyzed using LC-MS/MS technology. After they were applied to aging mice induced with D-gal, the skin hydration ability, antioxidant ability, collagen synthesis, and degradation ability of the mice were studied. The results show that FCP and NCP are mainly peptides that constitute type Ⅰ collagen, and their peptide segments are different. In vivo experiments show that FCP and NCP can improve the richness of collagen fibers in the skin of aging mice; improve the hydration ability of skin; promote the activity of antioxidant-related enzymes; and also show that through the TGF-β and MAPK pathways, the synthesis and degradation of collagen in skin are regulated. These results show that deer bone collagen peptide can improve skin problems caused by aging, promote skin hydration and antioxidant capacity of aging mice, and regulate collagen synthesis and degradation through the MAPK pathway.
摘要：
衰老引起的皮肤问题备受关注，海洋胶原蛋白肽已被证明可以改善这些问题，而哺乳动物胶原肽鲜有报道。在这项研究中,从发酵和非发酵鹿骨中提取发酵鹿骨胶原肽（FCP）和非发酵鹿骨胶原肽（NCP），分别,并使用LC-MS/MS技术分析了它们的肽序列和差异蛋白。将它们应用于D-gal诱导的衰老小鼠后，皮肤的水合能力，抗氧化能力，胶原蛋白合成，并对小鼠的降解能力进行了研究。结果表明,FCP和NCP主要是构成Ⅰ型胶原的多肽,和它们的肽段是不同的。体内实验表明，FCP和NCP可以提高衰老小鼠皮肤胶原纤维的丰富度；提高皮肤的水化能力；促进抗氧化相关酶的活性；还表明通过TGF-β和MAPK通路，皮肤胶原蛋白的合成和降解受到调控。这些结果表明，鹿骨胶原蛋白肽可以改善老化引起的皮肤问题，促进衰老小鼠的皮肤水合作用和抗氧化能力，并通过MAPK通路调节胶原蛋白的合成和降解。
