PDF(Pubmed)
Abstract：
Although glaucoma is a leading cause of irreversible blindness worldwide, its pathogenesis is incompletely understood, and intraocular pressure (IOP) is the only modifiable risk factor to target the disease. Several associations between the gut microbiome and glaucoma, including the IOP, have been suggested. There is growing evidence that interactions between microbes on the ocular surface, termed the ocular surface microbiome (OSM), and tear proteins, collectively called the tear proteome, may also play a role in ocular diseases such as glaucoma. This study aimed to find characteristic features of the OSM and tear proteins in patients with glaucoma. The whole-metagenome shotgun sequencing of 32 conjunctival swabs identified Actinobacteria, Firmicutes, and Proteobacteria as the dominant phyla in the cohort. The species Corynebacterium mastitidis was only found in healthy controls, and their conjunctival microbiomes may be enriched in genes of the phospholipase pathway compared to glaucoma patients. Despite these minor differences in the OSM, patients showed an enrichment of many tear proteins associated with the immune system compared to controls. In contrast to the OSM, this emphasizes the role of the proteome, with a potential involvement of immunological processes in glaucoma. These findings may contribute to the design of new therapeutic approaches targeting glaucoma and other associated diseases.
摘要：
虽然青光眼是全球不可逆失明的主要原因，其发病机制尚未完全了解，眼内压（IOP）是唯一可改变的危险因素。肠道微生物组和青光眼之间的几个关联，包括IOP,有人建议。越来越多的证据表明，眼表微生物之间的相互作用，称为眼表微生物组(OSM)，和眼泪蛋白，统称为眼泪蛋白质组，也可能在青光眼等眼部疾病中发挥作用。本研究旨在寻找青光眼患者OSM和泪液蛋白的特征性特征。32个结膜拭子的全基因组shot弹枪测序鉴定了放线菌，Firmicutes,和变形杆菌作为队列中的优势门。该物种只在健康对照中发现，与青光眼患者相比，它们的结膜微生物组可能富含磷脂酶途径的基因。尽管OSM有这些微小的差异，与对照组相比，患者表现出与免疫系统相关的许多泪液蛋白的富集。与OSM相比，这强调了蛋白质组的作用，与青光眼的免疫过程的潜在参与。这些发现可能有助于设计针对青光眼和其他相关疾病的新治疗方法。
