PDF(Pubmed)
Abstract：
Obese patients with asthma present with aggravated symptoms that are also harder to treat. Here, we used a mouse model of allergic asthma sensitised and challenged to house dust mite (HDM) extracts to determine whether high-fat-diet consumption would exacerbate the key features of allergic airway inflammation. C57BL/6 mice were intranasally sensitised and challenged with HDM extracts over a duration of 3 weeks. The impact of high-fat-diet (HFD) vs. normal diet (ND) chow was studied on HDM-induced lung inflammation and inflammatory cell infiltration as well as cytokine production. HFD-fed mice had greater inflammatory cell infiltration around airways and blood vessels, and an overall more severe degree of inflammation than in the ND-fed mice (semiquantitative blinded evaluation). Quantitative assessment of HDM-associated Th2 responses (numbers of lung CD4+ T cells, eosinophils, serum levels of allergen-specific IgE as well as the expression of Th2 cytokines (Il5 and Il13)) did not show significant changes between the HFD and ND groups. Interestingly, the HFD group exhibited a more pronounced neutrophilic infiltration within their lung tissues and an increase in non-Th2 cytokines (Il17, Tnfa, Tgf-b, Il-1b). These findings provide additional evidence that obesity triggered by a high-fat-diet regimen may exacerbate asthma by involving non-Th2 and neutrophilic pathways.
摘要：
肥胖的哮喘患者表现出加重的症状，也更难治疗。这里,我们使用对房尘螨(HDM)提取物致敏和挑战的过敏性哮喘小鼠模型来确定高脂饮食摄入是否会加剧过敏性气道炎症的关键特征.将C57BL/6小鼠鼻内致敏并用HDM提取物攻击3周的持续时间。高脂饮食（HFD）与研究了正常饮食（ND）食物对HDM诱导的肺部炎症和炎症细胞浸润以及细胞因子产生的影响。HFD喂养的小鼠气道和血管周围有更大的炎症细胞浸润，和总体上比ND喂养的小鼠更严重的炎症程度（半定量盲法评估）。HDM相关Th2反应的定量评估（肺CD4+T细胞数，嗜酸性粒细胞,血清过敏原特异性IgE水平以及Th2细胞因子（Il5和Il13）的表达在HFD和ND组之间没有显着变化。有趣的是,HFD组在其肺组织内表现出更明显的嗜中性粒细胞浸润和非Th2细胞因子的增加(Il17，Tnfa，Tgf-b,伊尔-1b)。这些发现提供了额外的证据，表明由高脂饮食方案引发的肥胖可能通过涉及非Th2和嗜中性粒细胞途径而加剧哮喘。
