PDF(Pubmed)
Abstract：
Curcumin is a natural compound that is considered safe and may have potential health benefits; however, its poor stability and water insolubility limit its therapeutic applications. Different strategies aim to increase its water solubility. Here, we tested the compound PVP-curcumin as a photosensitizer for antimicrobial photodynamic therapy (aPDT) as well as its potential to act as an adjuvant in antibiotic drug therapy. Gram-negative E. coli K12 and Gram-positive S. capitis were subjected to aPDT using various PVP-curcumin concentrations (1-200 µg/mL) and 475 nm blue light (7.5-45 J/cm2). Additionally, results were compared to aPDT using 415 nm blue light. Gene expression of recA and umuC were analyzed via RT-qPCR to assess effects on the bacterial SOS response. Further, the potentiation of Ciprofloxacin by PVP-curcumin was investigated, as well as its potential to prevent the emergence of antibiotic resistance. Both bacterial strains were efficiently reduced when irradiated with 415 nm blue light (2.2 J/cm2) and 10 µg/mL curcumin. Using 475 nm blue light, bacterial reduction followed a biphasic effect with higher efficacy in S. capitis compared to E. coli K12. PVP-curcumin decreased recA expression but had limited effect regarding enhancing antibiotic treatment or impeding resistance development. PVP-curcumin demonstrated effectiveness as a photosensitizer against both Gram-positive and Gram-negative bacteria but did not modulate the bacterial SOS response.
摘要：
姜黄素是一种天然化合物，被认为是安全的，可能具有潜在的健康益处;然而，其稳定性差和水不溶性限制了其治疗应用。不同的策略旨在增加其水溶性。这里,我们测试了化合物PVP-姜黄素作为抗菌光动力疗法(aPDT)的光敏剂,以及其在抗生素药物治疗中作为佐剂的潜力.使用各种PVP-姜黄素浓度（1-200μg/mL）和475nm蓝光（7.5-45J/cm2）对革兰氏阴性大肠杆菌K12和革兰氏阳性链球菌进行aPDT。此外,结果与使用415nm蓝光的aPDT进行比较。通过RT-qPCR分析recA和umuC的基因表达以评估对细菌SOS应答的影响。Further,研究了PVP-姜黄素对环丙沙星的增强作用，以及其防止抗生素耐药性出现的潜力。当用415nm蓝光（2.2J/cm2）和10μg/mL姜黄素照射时，两种细菌菌株均有效还原。使用475nm蓝光，与大肠杆菌K12相比,在头螺旋杆菌炎中具有更高的疗效。PVP-姜黄素降低recA表达，但在增强抗生素治疗或阻碍耐药性发展方面效果有限。PVP-姜黄素证明了作为光敏剂对革兰氏阳性和革兰氏阴性细菌的有效性，但不调节细菌SOS反应。
