PDF(Pubmed)
Abstract：
Skeletal muscle grows in response to a combination of genetic and environmental factors, and its growth and development influence the quality of pork. Elucidating the molecular mechanisms regulating the growth and development of skeletal muscle is of great significance to both animal husbandry and farm management. The Jiangquan black pig is an excellent pig breed based on the original Yimeng black pig, importing the genes of the Duroc pig for meat traits, and cultivated through years of scientific selection and breeding. In this study, full-length transcriptome sequencing was performed on three growth stages of Jiangquan black pigs, aiming to study the developmental changes in Jiangquan black pigs at different developmental stages at the molecular level and to screen the key genes affecting the growth of skeletal muscle in Jiangquan black pigs. We performed an enrichment analysis of genes showing differential expression and constructed a protein-protein interaction network with the aim of identifying core genes involved in the development of Jiangquan black pigs. Notably, genes such as TNNI2, TMOD4, PLDIM3, MYOZ1, and MYH1 may be potential regulators of muscle development in Jiangquan black pigs. Our results contribute to the understanding of the molecular mechanisms of skeletal muscle development in this pig breed, which will facilitate molecular breeding efforts and the development of pig breeds to meet the needs of the livestock industry.
摘要：
骨骼肌生长响应遗传和环境因素的组合，其生长发育影响着猪肉的品质。阐明调节骨骼肌生长发育的分子机制对畜牧业和农场管理都具有重要意义。江泉黑猪是在原沂蒙黑猪的基础上发展起来的优良猪种,导入杜洛克猪的基因以获得肉类性状，并通过多年的科学选择和育种培育。在这项研究中,对江泉黑猪的三个生长阶段进行了全长转录组测序，目的在分子水平上研究江泉黑猪不同发育阶段的发育变化，筛选影响江泉黑猪骨骼肌生长的关键基因。我们对表现出差异表达的基因进行了富集分析，并构建了蛋白质-蛋白质相互作用网络，旨在鉴定与江泉黑猪发育有关的核心基因。值得注意的是,TNI2、TMOD4、PLDIM3、MYOZ1和MYH1等基因可能是江泉黑猪肌肉发育的潜在调控因子。我们的结果有助于了解该猪品种骨骼肌发育的分子机制，这将促进分子育种工作和猪品种的发展，以满足畜牧业的需求。
