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Abstract：
The impact of gut and oral microbiota on the clinical outcomes of patients with oral squamous cell carcinoma (OSCC) is unknown. We compared the bacterial composition of dental plaque and feces between patients with OSCC and healthy controls (HCs). Fecal and dental plaque samples were collected from 7 HCs and 18 patients with OSCC before treatment initiation. Terminal restriction fragment-length polymorphism analysis of 16S rRNA genes was performed. Differences in bacterial diversity between the HC and OSCC groups were examined. We compared the occupancy of each bacterial species in samples taken from patients with OSCC and HCs and analyzed the correlation between PD-L1 expression in the tumor specimens and the occupancy of each bacterial species. The gut and oral microbiota of patients with OSCC were more varied than those of HCs. Porphyromonas and Prevotella were significantly more abundant in patients with OSCC than in HCs. The abundance of Clostridium subcluster XIVa in the gut microbiota of the PD-L1-positive group was significantly greater than that in the PD-L1-negative group. The oral and gut microbiomes of patients with OSCC were in a state of dysbiosis. Our results suggest the possibility of new cancer therapies targeting these disease-specific microbiomes using probiotics and synbiotics.
摘要：
肠道和口腔微生物群对口腔鳞状细胞癌（OSCC）患者临床结局的影响尚不清楚。我们比较了OSCC患者和健康对照（HCs）之间牙菌斑和粪便的细菌组成。在治疗开始前，从7例HC和18例OSCC患者收集粪便和牙菌斑样品。进行了16SrRNA基因的末端限制性片段长度多态性分析。检查了HC和OSCC组之间细菌多样性的差异。我们比较了取自OSCC和HC患者的样品中每种细菌物种的占有率，并分析了肿瘤标本中PD-L1表达与每种细菌物种占有率之间的相关性。OSCC患者的肠道和口腔微生物区比HC更多样化。OSCC患者的卟啉单胞菌和Prevotella明显高于HC。PD-L1阳性组肠道微生物群中梭菌亚簇XIVa的丰度显著高于PD-L1阴性组。OSCC患者的口腔和肠道微生物群处于菌群失调状态。我们的结果表明，使用益生菌和合生元针对这些疾病特异性微生物组的新癌症疗法的可能性。
