PDF(Pubmed)
Abstract：
Breeding salt-tolerant crops is necessary to reduce food insecurity. Prebreeding populations are fundamental for uncovering tolerance alleles from wild germplasm. To obtain a physiological interpretation of the agronomic salt tolerance and better criteria to identify candidate genes, quantitative trait loci (QTLs) governing productivity-related traits in a population of recombinant inbred lines (RIL) derived from S. pimpinellifolium were reanalyzed using an SNP-saturated linkage map and clustered using QTL meta-analysis to synthesize QTL information. A total of 60 out of 85 QTLs were grouped into 12 productivity MQTLs. Ten of them were found to overlap with other tomato yield QTLs that were found using various mapping populations and cultivation conditions. The MQTL compositions showed that fruit yield was genetically associated with leaf water content. Additionally, leaf Cl- and K+ contents were related to tomato productivity under control and salinity conditions, respectively. More than one functional candidate was frequently found, explaining most productivity MQTLs, indicating that the co-regulation of more than one gene within those MQTLs might explain the clustering of agronomic and physiological QTLs. Moreover, MQTL1.2, MQTL3 and MQTL6 point to the root as the main organ involved in increasing productivity under salinity through the wild allele, suggesting that adequate rootstock/scion combinations could have a clear agronomic advantage under salinity.
摘要：
培育耐盐作物对于减少粮食不安全是必要的。预交种群是从野生种质中发现耐受性等位基因的基础。为了获得农艺耐盐性的生理解释和更好的鉴定候选基因的标准，使用SNP饱和连锁图重新分析了源自木薯的重组自交系（RIL）种群中控制生产力相关性状的数量性状基因座（QTL），并使用QTL荟萃分析进行聚类以合成QTL信息。85个QTL中的60个被分为12个生产率MQTL。发现其中十个与使用各种作图种群和栽培条件发现的其他番茄产量QTL重叠。MQTL组成表明，果实产量与叶片含水量在遗传上相关。此外,在控制和盐度条件下，叶片Cl-和K含量与番茄生产力有关，分别。经常发现不止一个功能候选，解释大多数生产力MQTL，表明这些MQTL中多个基因的共调控可能解释了农艺和生理QTL的聚类。此外，MQTL1.2，MQTL3和MQTL6通过野生等位基因指向根作为参与盐度下生产力提高的主要器官，表明在盐度下，足够的砧木/接穗组合可能具有明显的农艺优势。
