PDF(Pubmed)
Abstract：
Transcription factors are key molecules involved in transcriptional and post-transcriptional regulation in plants and play an important regulatory role in resisting biological stress. In this study, we identified a regulatory factor, OsZF8, mediating rice response to Rhizoctonia solani (R. solani) AG1-IA infection. The expression of OsZF8 affects R. solani rice infection. OsZF8 knockout and overexpressed rice plants were constructed, and the phenotypes of mutant and wild-type (WT) plants showed that OsZF8 negatively regulated rice resistance to rice sheath blight. However, it was speculated that OsZF8 plays a regulatory role at the protein level. The interacting protein PRB1 of OsZF8 was screened using the yeast two-hybrid and bimolecular fluorescence complementation test. The results showed that OsZF8 effectively inhibited PRB1-induced cell death in tobacco cells, and molecular docking results showed that PRB1 had a strong binding effect with OsZF8. Further, the binding ability of OsZF8-PRB1 to ergosterol was significantly reduced when compared with the PRB1 protein. These findings provide new insights into elucidating the mechanism of rice resistance to rice sheath blight.
摘要：
转录因子是参与植物转录和转录后调控的关键分子，在抵抗生物胁迫中起着重要的调节作用。在这项研究中,我们确定了一个调节因素，OsZF8，介导水稻对枯萎病菌的反应（R。solani)AG1-IA感染。OsZF8的表达影响水稻感染。构建了OsZF8基因敲除和过表达的水稻植株，突变体和野生型（WT）植物的表型表明，OsZF8负调控水稻对水稻纹枯病的抗性。然而,推测OsZF8在蛋白质水平起调节作用。利用酵母双杂交和双分子荧光互补试验筛选了OsZF8的相互作用蛋白PRB1。结果表明,OsZF8能有效抑制PRB1诱导的烟草细胞死亡,分子对接结果表明,PRB1与OsZF8有较强的结合作用。Further,与PRB1蛋白相比，OsZF8-PRB1与麦角甾醇的结合能力显着降低。这些发现为阐明水稻对水稻纹枯病的抗性机制提供了新的见解。
