PDF(Pubmed)
Abstract：
In the cosmetics industry, the extract from Raphanus sativus L. is fermented using specific starter cultures. These cosmetic ingredients act as preservatives and skin conditioners. Kombucha is traditionally made by fermenting sweetened tea using symbiotic cultures of bacteria and yeast and is used in cosmetic products. The aim of this study was to evaluate the cosmetic properties of radish leaf and root extract fermented with the SCOBY. Both unfermented water extracts and extracts after 7, 14, and 21 days of fermentation were evaluated. The analysis of secondary plant metabolites by UPLC-MS showed higher values for ferments than for extracts. A similar relationship was noted when examining the antioxidant properties using DPPH and ABTS radicals and the protective effect against H2O2-induced oxidative stress in fibroblasts and keratinocytes using the fluorogenic dye H2DCFDA. The results also showed no cytotoxicity to skin cells using Alamar Blue and Neutral Red tests. The ability of the samples to inhibit IL-1β and COX-2 activity in LPS-treated fibroblasts was also demonstrated using ELISA assays. The influence of extracts and ferments on bacterial strains involved in inflammatory processes of skin diseases was also assessed. Additionally, application tests were carried out, which showed a positive effect of extracts and ferments on TEWL and skin hydration using a TEWAmeter and corneometer probe. The results obtained depended on the concentration used and the fermentation time.
摘要：
在化妆品行业，RaphanussativusL.的提取物使用特定的发酵剂进行发酵。这些化妆品成分充当防腐剂和皮肤调理剂。红茶菌传统上是通过使用细菌和酵母的共生培养物发酵甜茶制成的，用于化妆品。这项研究的目的是评估用SCOBY发酵的萝卜叶和根提取物的化妆品特性。评估未发酵的水提取物和发酵7、14和21天后的提取物。通过UPLC-MS对次生植物代谢物的分析显示，发酵剂的值高于提取物。当使用DPPH和ABTS自由基检查抗氧化特性以及使用荧光染料H2DCFDA对成纤维细胞和角质形成细胞中H2O2诱导的氧化应激的保护作用时，注意到了类似的关系。结果还显示使用Alamar蓝和中性红测试对皮肤细胞没有细胞毒性。样品在LPS处理的成纤维细胞中抑制IL-1β和COX-2活性的能力也用ELISA测定法证明。还评估了提取物和发酵液对参与皮肤病炎症过程的细菌菌株的影响。此外,进行了应用测试，使用TEWAmeter和角质计探针显示提取物和发酵液对TEWL和皮肤水合作用的积极作用。获得的结果取决于使用的浓度和发酵时间。
