PDF(Pubmed)
Abstract：
Over the years, oysters have faced recurring mass mortality issues during the summer breeding season, with Vibrio infection emerging as a significant contributing factor. Tubules of gill filaments were confirmed to be in the hematopoietic position in Crassostrea gigas, which produce hemocytes with immune defense capabilities. Additionally, the epithelial cells of oyster gills produce immune effectors to defend against pathogens. In light of this, we performed a transcriptome analysis of gill tissues obtained from C. gigas infected with Vibrio alginolyticus for 12 h and 48 h. Through this analysis, we identified 1024 differentially expressed genes (DEGs) at 12 h post-injection and 1079 DEGs at 48 h post-injection. Enrichment analysis of these DEGs revealed a significant association with immune-related Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. To further investigate the immune response, we constructed a protein-protein interaction (PPI) network using the DEGs enriched in immune-associated KEGG pathways. This network provided insights into the interactions and relationships among these genes, shedding light on the underlying mechanisms of the innate immune defense mechanism in oyster gills. To ensure the accuracy of our findings, we validated 16 key genes using quantitative RT-PCR. Overall, this study represents the first exploration of the innate immune defense mechanism in oyster gills using a PPI network approach. The findings provide valuable insights for future research on oyster pathogen control and the development of oysters with enhanced antimicrobial resistance.
摘要：
多年来,牡蛎在夏季繁殖季节面临反复出现的大规模死亡问题，弧菌感染成为一个重要的促成因素。在Crassostreagigas，确认了g丝的小管处于造血位置，产生具有免疫防御能力的血细胞。此外,牡蛎的上皮细胞产生免疫效应来防御病原体。鉴于此,我们对从溶藻弧菌感染12小时和48小时的C.gigas中获得的g组织进行了转录组分析。通过此分析，我们在注射后12h鉴定出1024个差异表达基因(DEGs),在注射后48h鉴定出1079个DEGs.这些DEGs的富集分析揭示了与免疫相关的基因本体论（GO）术语和京都基因和基因组百科全书（KEGG）途径的显着关联。为了进一步研究免疫反应，我们使用富含免疫相关KEGG途径的DEGs构建了蛋白质-蛋白质相互作用(PPI)网络.这个网络提供了对这些基因之间相互作用和关系的见解，揭示牡蛎g先天免疫防御机制的潜在机制。为了确保我们发现的准确性，我们使用定量RT-PCR验证了16个关键基因。总的来说,这项研究代表了首次使用PPI网络方法探索牡蛎的先天免疫防御机制。这些发现为未来牡蛎病原体控制和具有增强的抗菌素耐药性的牡蛎的发展提供了有价值的见解。
