PDF(Pubmed)
Abstract：
This study evaluated the effects of Pediococcus pentosaceus GT001 on Salmonella typhimurium (S. typhimurium)-challenged broiler chickens. Two hundred Ross 708 broiler day-old chicks with comparable weight were distributed at random into four treatments with five replicates and ten chicks per replicate. The following were the treatment groups: (B) basal diet (control); (B + S) basal diet and birds were challenged with S. typhimurium at 1.0 × 107 cfu/g; (B + P) basal diet + Pediococcus pentosaceus GT001 at 4.0 × 108 cfu/g; (B + P + S) basal diet + P. pentosaceus GT001 at 4.0 × 108 cfu/g and birds were challenged with S. typhimurium at 1.0 × 107 cfu/g. There was a significant reduction (p < 0.05) in the body weight of the Salmonella-infected birds compared to the other treatment groups. However, the FCRs of the broilers were comparable among the different treatment groups (p > 0.05). The lipid profile and liver function indices measured were significantly enhanced in the P. pentosaceus GT001-supplemented groups (B + P and B + P + S) compared to the group that was Salmonella-challenged (p < 0.05) but were similar to those in the control group. The serum antioxidant activities, such as the T-AOC, SOD, CAT, GHS-Px and MDA, were significantly improved in the P. pentosaceus GT001-supplemented groups (B + P and B + P + S) (p < 0.05). The MDA was similar in the B + P and B + P + S groups, but both were significantly lower than the control and the Salmonella groups. The administration of P. pentosaceus GT001 enhanced the lipase and amylase levels in both the serum and intestine of the broilers (p < 0.05). The immunoglobin (IgA, IgG, IgM) and cytokine (IL-10 and IL-6) levels in the serum were significantly higher in the B, B + P and B + P + S treatment groups (p < 0.05). The immune-related organs (bursa and spleen) were significantly influenced in the birds fed with P. pentosaceus GT001. No significant variation was noted among all the dietary treatments in terms of the measured meat quality indices. The small intestinal digesta content of the Salmonella load was below a detectable range after 14 days of infection (p < 0.05). No significant differences were observed among the different treatment groups in terms of the breast pH, drip loss and meat color (p > 0.05). The inclusion of P. pentosaceus GT001 also modified the community structure in the cecum. This indicates that it has health benefits and could be incorporated in the broiler diet.
摘要：
本研究评估了戊糖片球菌GT001对鼠伤寒沙门氏菌（S.鼠伤寒）攻击的肉鸡。将200只具有相当体重的Ross708日龄肉鸡随机分配到四个处理中，每个重复五个重复和十个小鸡。以下是治疗组：（B）基础饮食（对照）；（BS）基础饮食和鸟类用1.0×107cfu/g的鼠伤寒沙门氏菌攻击；（BP）基础饮食，戊糖片GT001为4.0×108cfu/g；（BPS）基础饮食，戊糖片GT001为4.0×108cfu/g，禽类为1.0。与其他处理组相比,沙门氏菌感染的禽类的体重显著降低(p<0.05)。然而,不同处理组的肉仔鸡FCR具有可比性（p>0.05）。与沙门氏菌攻击组(p<0.05)相比,在补充戊糖疟原虫GT001组(B+P和B+P+S)中测得的脂质特征和肝功能指标显著增强,但与对照组相似。血清抗氧化活性，比如T-AOC,SOD,CAT,GHS-Px和MDA,在添加了PantosacusGT001的组中（BP和BPS）显着改善（p<0.05）。MDA在B+P和B+P+S组相似，但两者均显着低于对照组和沙门氏菌组。给予戊糖牛GT001可提高肉鸡血清和肠道中的脂肪酶和淀粉酶水平(p<0.05)。免疫球蛋白（IgA，IgG,血清中IgM)和细胞因子(IL-10和IL-6)程度显著高于B,B+P和B+P+S医治组(p<0.05)。在饲喂戊糖GT001的鸟类中，免疫相关器官（法氏囊和脾脏）受到显着影响。就测得的肉质指数而言，在所有饮食处理中都没有发现显着差异。感染14天后,沙门氏菌负荷的小肠消化物含量低于可检测范围(p<0.05)。不同治疗组的乳腺pH值无显著差异,滴水损失和肉色(p>0.05)。pososacusGT001的包含也改变了盲肠中的群落结构。这表明它具有健康益处，可以纳入肉鸡饮食中。
