PDF(Pubmed)
Abstract：
The aim of this study was to investigate the interferon tau (IFNt) concentration in the peripheral maternal blood during the early phase of pregnancy in buffalo cows and improve the knowledge on the physiological importance of circulating IFNt, evaluating the possible interaction with pregnancy-associated glycoproteins (PAGs) and progesterone (P4). Blood samples were taken from buffalo cows on day 0 (day of AI), 7, 14, 18, 28, and 40 post insemination for the IFNt, PAG, and P4 analysis and to determine the IFNt mRNA expression. The animals were categorized ex post into Pregnant, Non-pregnant and Embryo mortality groups. The interferon value was influenced by group (p = 0.003), being always higher in pregnant buffalo cows than in non-pregnant ones, while the embryo mortality group showed intermediate values between those for pregnant and non-pregnant animals. The mRNA expression of IFNt was not influenced by groups or any time points. The regression analysis that included IFNt as the independent variable showed that PAGs, from day 18 (p < 0.01), and P4, from day 28 (p < 0.05), were positively associated with IFNt values. The close associations among IFNt, PAGs and P4 demonstrate that all three molecules work together for fetal-placental well-being and pregnancy support. Unfortunately, the great individual variability in circulating IFNt makes this analysis unsuitable for early pregnancy diagnosis.
摘要：
本研究的目的是调查水牛妊娠早期外周血中干扰素tau（IFNt）的浓度，并提高对循环IFNt的生理重要性的认识。评估与妊娠相关糖蛋白（PAG）和孕酮（P4）的可能相互作用。在第0天（AI天）从水牛中采集血液样本，IFNt的7、14、18、28和40后授精，PAG,和P4分析并确定IFNtmRNA的表达。这些动物在事后被归类为怀孕，非妊娠和胚胎死亡率组。干扰素值受影响组（p=0.003），怀孕的水牛总是比非怀孕的水牛高，而胚胎死亡率组显示在怀孕和非怀孕动物之间的中间值。IFNt的mRNA表达不受组或任何时间点的影响。以IFNt为自变量的回归分析表明，PAG、从第18天开始（p<0.01），和P4，从第28天开始（p<0.05），与IFNt值呈正相关。IFNt之间的紧密联系，PAG和P4表明所有三种分子共同作用于胎儿-胎盘健康和妊娠支持。不幸的是,循环IFNt的巨大个体差异使得该分析不适用于早期妊娠诊断.
