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Abstract：
Pasteurellosis, a disease caused by Pasteurella multocida, is responsible for economic losses in rabbit industrial farms due to rhinitis, conjunctivitis, pneumonia, metritis, mastitis, orchitis, subcutaneous abscesses, otitis, encephalitis, and septicaemic forms. Although the occurrence of the disease is conditioned by predisposing factors that affect the rabbit immune response, the strains of P. multocida involved in the infection may have a different pathogenic ability. Therefore, typing of strains spread among the rabbits is important to assess their pathogenic potential. The aim of this study is to investigate the P. multocida strains responsible for disease in rabbit industrial farms. A total of 114 strains identified from different lesions were serotyped. Additionally, the presence of virulence-associated genes was investigated using three PCR (polymerase chain reaction) protocols. Capsular type A was prevalently found in strains from respiratory lesions while types D and F in those from metritis, mastitis, and other lesions. Different associations between some virulence-associated genes and both capsular type and lesions found in rabbits were detected. The presence of 8 virulence-associated genes seems to increase the occurrence of metritis. In addition, strains belonging to capsular type A and responsible for respiratory disorders especially, were found equipped with 10 and 11 virulence-associated genes. Nevertheless, the presence of strains responsible only for rhinitis was also detected among the latter, suggesting that the pathogenic ability of the bacteria depends on the expression rather than the presence of a gene.
摘要：
巴氏杆菌病,一种由多杀巴斯德氏菌引起的疾病，负责由于鼻炎造成的兔子工业农场的经济损失，结膜炎,肺炎,子宫炎,乳腺炎,睾丸炎,皮下脓肿,中耳炎,脑炎,和败血症形式。尽管该病的发生受到影响兔免疫反应的诱发因素的制约,参与感染的多杀性疟原虫菌株可能具有不同的致病能力。因此,在兔子中传播的菌株的分型对于评估其致病潜力很重要。这项研究的目的是调查在兔工业农场中引起疾病的多杀性疟原虫菌株。对来自不同病变的总共114个菌株进行了血清分型。此外,使用三种PCR(聚合酶链反应)方案研究了毒力相关基因的存在.在呼吸道病变的菌株中普遍发现A型囊，而在子宫炎的菌株中普遍发现D型和F型囊。乳腺炎,和其他病变。检测到一些毒力相关基因与兔子中发现的荚膜类型和病变之间的不同关联。8个毒力相关基因的存在似乎增加了子宫炎的发生。此外,属于荚膜A型的菌株，尤其是负责呼吸系统疾病的菌株，被发现装有10和11个毒力相关基因。然而，在后者中也检测到仅对鼻炎负责的菌株的存在，这表明细菌的致病能力取决于基因的表达而不是基因的存在。
