PDF(Pubmed)
Abstract：
Studies on obligate halophytes combining eco-physiological techniques and proteomic analysis are crucial for understanding salinity tolerance mechanisms but are limited. We thus examined growth, water relations, ion homeostasis, photosynthesis, oxidative stress mitigation and proteomic responses of an obligate halophyte Suaeda fruticosa to increasing salinity under semi-hydroponic culture. Most biomass parameters increased under moderate (300 mmol L-1 of NaCl) salinity, while high (900 mmol L-1 of NaCl) salinity caused some reduction in biomass parameters. Under moderate salinity, plants showed effective osmotic adjustment with concomitant accumulation of Na+ in both roots and leaves. Accumulation of Na+ did not accompany nutrient deficiency, damage to photosynthetic machinery and oxidative damage in plants treated with 300 mmol L-1 of NaCl. Under high salinity, plants showed further decline in sap osmotic potential with higher Na+ accumulation that did not coincide with a decline in relative water content, Fv/Fm, and oxidative damage markers (H2O2 and MDA). There were 22, 54 and 7 proteins in optimal salinity and 29, 46 and 8 proteins in high salinity treatment that were up-regulated, down-regulated or exhibited no change, respectively, as compared to control plants. These data indicate that biomass reduction in S. fruticosa at high salinity might result primarily from increased energetic cost rather than ionic toxicity.
摘要：
结合生态生理技术和蛋白质组学分析的专性盐生植物的研究对于理解盐分耐性机制至关重要，但有限。因此，我们检查了增长，水关系,离子稳态，光合作用,在半水培培养下，专性盐生植物碱蓬对盐度增加的氧化应激缓解和蛋白质组学反应。大多数生物量参数在中等盐度（300mmolL-1的NaCl）下增加，而高盐度（900mmolL-1的NaCl）导致生物量参数有所下降。在中等盐度下，植物表现出有效的渗透调节，并伴随着Na在根和叶中的积累。Na+的积累并不伴随营养缺乏，用300mmolL-1的NaCl处理对植物的光合机制和氧化损伤。在高盐度下，植物显示树液渗透势进一步下降，Na积累增加，这与相对含水量的下降并不一致，Fv/Fm,和氧化损伤标志物(H2O2和MDA)。在最佳盐度处理中有22、54和7种蛋白质，在高盐度处理中有29、46和8种蛋白质被上调，下调或没有变化，分别,与对照植物相比。这些数据表明,在高盐度条件下,fruitcosa中的生物量减少可能主要是由于增加的能量成本而不是离子毒性。
