PDF(Pubmed)
Abstract：
Graft healing is a complex process affected by environmental factors, with temperature being one of the most important influencing factors. Here, oriental melon grafted onto pumpkin was used to study changes in graft union formation and sugar contents at the graft interface under night temperatures of 18 °C and 28 °C. Histological analysis suggested that callus formation occurred 3 days after grafting with a night temperature of 28 °C, which was one day earlier than with a night temperature of 18 °C. Vascular reconnection with a night temperature of 28 °C was established 2 days earlier than with a night temperature of 18 °C. Additionally, nine sugars were significantly enriched in the graft union, with the contents of sucrose, trehalose, raffinose, D-glucose, D-fructose, D-galactose, and inositol initially increasing but then decreasing. Furthermore, we also found that exogenous glucose and fructose application promotes vascular reconnection. However, exogenous sucrose application did not promote vascular reconnection. Taken together, our results reveal that elevated temperatures improve the process of graft union formation through increasing the contents of sugars. This study provides information to develop strategies for improving grafting efficiency under low temperatures.
摘要：
移植物愈合是一个受环境因素影响的复杂过程,温度是最重要的影响因素之一。这里,在18°C和28°C的夜间温度下，嫁接到南瓜上的东方甜瓜用于研究嫁接结合形成和嫁接界面糖分含量的变化。组织学分析表明，在28°C的夜间温度下，嫁接后3天发生了愈伤组织的形成，比夜间温度18°C早一天。夜间温度为28°C的血管重新连接比夜间温度为18°C的时间早2天。此外,九种糖在嫁接结合中显著富集，蔗糖的含量，海藻糖,棉子糖,D-葡萄糖,D-果糖,D-半乳糖,和肌醇最初增加但随后减少。此外，我们还发现外源性葡萄糖和果糖的应用促进血管再连接。然而,外源蔗糖的应用并不促进血管再连接。一起来看,我们的结果表明，升高的温度通过增加糖的含量改善了接枝结合形成的过程。本研究为开发低温下提高接枝效率的策略提供了信息。
