PDF(Pubmed)
Abstract：
The two accessions of the polytrichaceous moss species Polytrichum formosum, namely German and Serbian genotypes, were subjected to salt stress, aiming to study the species\' developmental and physiological features. Various concentrations of sodium chloride were applied to an axenic in vitro culture of the two moss genotypes, and the growth parameters as well as physiological feature changes were followed. As inferred by the morpho-developmental parameters and survival index, the Serbian genotype showed higher resistance to salt stress as compared to the German one. However, both moss genotypes survived the highest applied concentration (500 mM). As expected, short exposures to salt were rather easily overcome. No clear patterns in sugar content and changes were observed during the stress, but they are surely included in salt stress response and tolerance in P. formosum. Longer stress increased total chlorophyll content in both genotypes. In short-term applied salt stress, the Serbian genotype had a higher total chlorophyll concentration to control unstressed plants, while the German genotype decreased the total amount of chlorophyll. Similarly, carotenoids were shown to be significantly higher in the Serbian genotype, both in unstressed and treated plants, compared to the German one. The contents of tocopherols were higher in the Serbian genotype in controlled unstressed and subsequently short- and long-stressed plantlets compared to the German accession. In general, we can assume that P. formosum is unexpectedly tolerant to salt stress and that there are differences within various accessions of overall European populations, as referred by two randomly selected genotypes, which is most probably a consequence of different genetic structure.
摘要：
多枝苔藓种的两种种质，即德国和塞尔维亚基因型，受到盐胁迫，旨在研究该物种的发育和生理特征。将各种浓度的氯化钠应用于两种苔藓基因型的无菌体外培养物中，并跟踪生长参数和生理特征变化。根据形态发育参数和生存指数推断，与德国基因型相比，塞尔维亚基因型对盐胁迫的抵抗力更高。然而,两种苔藓基因型均在最高浓度（500mM）下存活。不出所料,短时间接触盐很容易克服。在胁迫期间没有观察到糖含量和变化的清晰模式，但它们肯定包括在Formosum的盐应激反应和耐受性中。更长的胁迫增加了两种基因型的总叶绿素含量。在短期施加的盐胁迫中，塞尔维亚基因型具有较高的总叶绿素浓度来控制未受胁迫的植物，而德国基因型降低了叶绿素的总量。同样，类胡萝卜素在塞尔维亚基因型中显示明显更高，在无应力和经过处理的植物中，与德国相比。与德国登录名相比，塞尔维亚基因型的生育酚含量更高，在受控的无胁迫和随后的短期和长期胁迫的小植株中。总的来说,我们可以假设Formosum对盐胁迫具有出乎意料的耐受性，并且在整个欧洲人口的各种种质中存在差异，由两个随机选择的基因型，这很可能是不同遗传结构的结果。
