PDF(Pubmed)
Abstract：
Temporomandibular disorders (TMDs) are a heterogeneous group of musculoskeletal and neuromuscular conditions involving the temporomandibular joint (TMJ), masticatory muscles, and associated structures. Mesenchymal stromal/stem cells (MSCs) have emerged as a promising therapy for TMJ repair. This systematic review aims to consolidate findings from the preclinical animal studies evaluating MSC-based therapies, including MSCs, their secretome, and extracellular vesicles (EVs), for the treatment of TMJ cartilage/osteochondral defects and osteoarthritis (OA). Following the PRISMA guidelines, PubMed, Embase, Scopus, and Cochrane Library databases were searched for relevant studies. A total of 23 studies involving 125 mice, 149 rats, 470 rabbits, and 74 goats were identified. Compliance with the ARRIVE guidelines was evaluated for quality assessment, while the SYRCLE risk of bias tool was used to assess the risk of bias for the studies. Generally, MSC-based therapies demonstrated efficacy in TMJ repair across animal models of TMJ defects and OA. In most studies, animals treated with MSCs, their derived secretome, or EVs displayed improved morphological, histological, molecular, and behavioral pain outcomes, coupled with positive effects on cellular proliferation, migration, and matrix synthesis, as well as immunomodulation. However, unclear risk in bias and incomplete reporting highlight the need for standardized outcome measurements and reporting in future investigations.
摘要：
颞下颌关节紊乱病（TMD）是一组涉及颞下颌关节（TMJ）的肌肉骨骼和神经肌肉疾病，咀嚼肌,和相关的结构。间充质基质/干细胞（MSC）已成为TMJ修复的有希望的疗法。本系统综述旨在巩固评估基于MSC疗法的临床前动物研究的结果。包括MSC,他们的秘密,和细胞外囊泡（EV），用于治疗TMJ软骨/骨软骨缺损和骨关节炎（OA）。按照PRISMA准则，PubMed,Embase,Scopus,和Cochrane图书馆数据库进行了相关研究。共有23项研究涉及125只小鼠，149只老鼠,470只兔子确定了74只山羊。对ARRIVE指南的合规性进行了质量评估，而SYRCLE偏倚风险工具用于评估研究偏倚风险。一般来说,基于MSC的疗法在TMJ缺损和OA的动物模型中表现出TMJ修复的功效。在大多数研究中,用MSC治疗的动物，它们衍生的分泌组，或电动汽车显示出改善的形态学，组织学,分子，和行为疼痛的结果，加上对细胞增殖的积极影响，迁移,和矩阵合成，以及免疫调节。然而,偏倚风险不明确和报告不完整凸显了在未来调查中需要标准化的结局测量和报告.
