PDF(Pubmed)
Abstract：
The ubiquitous presence of antimicrobial-resistant organisms and antimicrobial resistance genes (ARGs) constitutes a major threat to global public safety. Tetracycline (TET) is a common antimicrobial agent that inhibits bacterial growth and is frequently detected in aquatic environments. Although TET may display coselection for resistance, limited knowledge is available on whether and how it might influence plasmid-mediated conjugation. Subinhibitory concentrations (3.9-250 ng/mL) of TET promoted horizontal gene transfer (HGT) via the mobilizable plasmid pVP52-1 from the donor Vibrio parahaemolyticus NJIFDCVp52 to the recipient Escherichia coli EC600 by 1.47- to 3.19-fold. The transcription levels of tetracycline resistance genes [tetA, tetR(A)], conjugation-related genes (traA, traD), outer membrane protein genes (ompA, ompK, ompV), reactive oxygen species (ROS)-related genes (oxyR, rpoS), autoinducer-2 (AI-2) synthesis gene (luxS), and SOS-related genes (lexA, recA) in the donor and recipient were significantly increased. Furthermore, the overproduced intracellular ROS generation and increased cell membrane permeability under TET exposure stimulated the conjugative transfer of ARGs. Overall, this study provides important insights into the contributions of TET to the spread of antimicrobial resistance.
摘要：
抗生素抗性生物和抗生素抗性基因(ARGs)的普遍存在对全球公共安全构成了重大威胁。四环素（TET）是一种常见的抗微生物剂，可抑制细菌生长，并在水生环境中经常检测到。尽管TET可能显示出抵抗的共选择，关于它是否以及如何影响质粒介导的接合的知识有限。亚抑制浓度（3.9-250ng/mL）的TET通过可动员的质粒pVP52-1从供体副溶血性弧菌NJIFDCVp52到受体大肠杆菌EC600的水平基因转移（HGT）增加了1.47至3.19倍。四环素抗性基因的转录水平[tetA，tetR(A)],共轭相关基因(traA，traD)，外膜蛋白基因(ompA，ompK,ompV),活性氧（ROS）相关基因（oxyR，rpos),自诱导-2（AI-2）合成基因（luxS），和SOS相关基因(lexA，recA)在捐赠者和接受者中显著增加。此外，TET暴露下过度产生的细胞内ROS产生和细胞膜通透性增加刺激了ARGs的共轭转移。总的来说,这项研究为TET对抗菌药物耐药性传播的贡献提供了重要的见解。
