PDF(Pubmed)
Abstract：
Herbal teas have attracted attention as functional beverages containing luteolin and apigenin, which exhibit antioxidant and anti-inflammatory effects. The objective of this study was to develop a sensitive online automated method to determine these flavones\' contents in herbal teas using in-tube solid-phase microextraction (IT-SPME) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). These compounds were extracted and concentrated by IT-SPME using a Supel Q PLOT capillary column and then separated and detected within 6 min using a CAPCELL PAK C18 MG III analytical column and a negative electrospray ionization-mode multiple-reaction monitoring system by LC-MS/MS. The detection limits (S/N = 3) for luteolin and apigenin were 0.4 and 0.8 pg mL-1, respectively, and the calibration curves were linear in the range of 2-2000 pg mL-1 with correlation coefficients above 0.9995, and intra-day and inter-day precisions with relative standard deviations below 2.9 and 3.6% (n = 6), respectively. The luteolin and apigenin in herbal tea were quantified using IT-SPME/LC-MS/MS following the acid hydrolysis of their glycosides. Among the 10 herbal teas tested, luteolin was detected in peppermint and sage at concentrations of 375 and 99 µg mL-1, respectively, while apigenin was detected in German chamomile at 110 µg mL-1, which were higher than in the other herbal teas. The method is expected to be a useful method for evaluating the efficacy of luteolin and apigenin in herbal teas as functional beverages.
摘要：
草药茶作为含有木犀草素和芹菜素的功能性饮料引起了人们的关注，表现出抗氧化和抗炎作用。本研究的目的是开发一种灵敏的在线自动方法，使用管内固相微萃取（IT-SPME）结合液相色谱-串联质谱（LC-MS/MS）测定草药茶中的黄酮含量。使用SupelQPLOT毛细管柱通过IT-SPME提取和浓缩这些化合物，然后在6分钟内使用CAPCELLPAKC18MGIII分析柱和通过LC-MS/MS的负电喷雾电离模式多反应监测系统进行分离和检测。木犀草素和芹菜素的检出限（S/N=3）分别为0.4和0.8pgmL-1，校准曲线在2-2000pgmL-1范围内呈线性关系，相关系数高于0.9995，日内和日间精度相对标准偏差低于2.9和3.6％（n=6），分别。在其糖苷酸水解后，使用IT-SPME/LC-MS/MS定量草药茶中的木犀草素和芹菜素。在测试的10种草药茶中，在薄荷和鼠尾草中检测到木犀草素的浓度分别为375和99µgmL-1，而芹菜素在德国洋甘菊中检测到110µgmL-1，高于其他草药茶。该方法有望成为评价木犀草素和芹菜素作为功能性饮料在草药茶中的功效的有用方法。
