PDF(Pubmed)
Abstract：
In advanced drug delivery, versatile liposomal formulations are commonly employed for safer and more accurate therapies. Here we report a method that allows a straightforward production of synthetic monodisperse (~ 100 μm) giant unilamellar vesicles (GUVs) using a microfluidic system. The stability analysis based on the microscopy imaging showed that at ambient conditions the produced GUVs had a half-life of 61 ± 2 h. However, it was observed that ~ 90% of the calcein dye that was loaded into GUVs was transported into a surrounding medium in 24 h, thus indicating that the GUVs may release these small dye molecules without distinguishable membrane disruption. We further demonstrated the feasibility of our method by loading GUVs with larger and very different cargo objects; small soluble fluorescent proteins and larger magnetic microparticles in a suspension. Compared to previously reported microfluidics-based production techniques, the obtained results indicate that our simplified method could be equally harnessed in creating GUVs with less cost, effort and time, which could further benefit studying closed membrane systems.
摘要：
在先进的药物输送中，通用脂质体制剂通常用于更安全和更准确的治疗。在这里，我们报告了一种方法，该方法允许使用微流体系统直接生产合成的单分散（〜100μm）巨型单层囊泡（GUV）。基于显微镜成像的稳定性分析表明，在环境条件下，生产的GUV的半衰期为61±2小时。据观察，约90％的钙黄绿素染料装载到GUV运输到周围的培养基在24小时，因此表明GUV可以释放这些小染料分子而没有可区分的膜破坏。我们进一步证明了我们的方法的可行性通过加载GUV更大和非常不同的货物对象;小可溶性荧光蛋白和较大的磁性微粒在悬浮液中。与以前报道的基于微流体的生产技术相比，获得的结果表明，我们的简化方法可以同样地利用在创建GUV时，成本更低，努力和时间,这可以进一步有利于研究封闭膜系统。
