Abstract：
OBJECTIVE: To determine the effects of prolonged administration of the oral NSAIDs phenylbutazone and firocoxib on concentrations of cytokines and growth factors in platelet-rich plasma (PRP) and autologous protein solution (APS).
METHODS: 6 adult University owned horses.
METHODS: Horses were randomized to receive phenylbutazone (1 g, orally, q 12 h) or firocoxib (57 mg, orally, q 24 h) for 6 days. Blood was obtained and processed for APS (Pro-Stride) and PRP (Restigen) before the administration of NSAIDs and at 7 days (1 day following cessation of NSAIDs). Horses underwent a two-week washout period, during which blood was obtained at 14 days and 21 days. The protocol was repeated with a crossover design. PRP and APS were analyzed for concentrations of platelets, leukocytes, and several cytokines (IL-1β, IL-10, IL-6, IL-8, and tumor necrosis factor-α) and growth factors (PDGF, FGF-2, and TGF-β1) using immunoassays. Plasma was evaluated for drug concentrations.
RESULTS: No significant differences existed in concentrations of growth factors and cytokines before or after prolonged administration of NSAIDs. There were significant differences in concentrations of leukocytes and platelets in PRP compared to APS, with higher concentrations of leukocytes at the day 7 time point (T) in APS (phenylbutazone) and in concentrations of platelets in APS at T0 (firocoxib) and in APS at T7 (phenylbutazone).
CONCLUSIONS: Veterinarians can recommend the administration of these oral NSAIDs prior to obtaining blood for PRP and APS provided a single-day washout period is instituted.
METHODS: 6 adult University owned horses.
METHODS: Horses were randomized to receive phenylbutazone (1 g, orally, q 12 h) or firocoxib (57 mg, orally, q 24 h) for 6 days. Blood was obtained and processed for APS (Pro-Stride) and PRP (Restigen) before the administration of NSAIDs and at 7 days (1 day following cessation of NSAIDs). Horses underwent a two-week washout period, during which blood was obtained at 14 days and 21 days. The protocol was repeated with a crossover design. PRP and APS were analyzed for concentrations of platelets, leukocytes, and several cytokines (IL-1β, IL-10, IL-6, IL-8, and tumor necrosis factor-α) and growth factors (PDGF, FGF-2, and TGF-β1) using immunoassays. Plasma was evaluated for drug concentrations.
RESULTS: No significant differences existed in concentrations of growth factors and cytokines before or after prolonged administration of NSAIDs. There were significant differences in concentrations of leukocytes and platelets in PRP compared to APS, with higher concentrations of leukocytes at the day 7 time point (T) in APS (phenylbutazone) and in concentrations of platelets in APS at T0 (firocoxib) and in APS at T7 (phenylbutazone).
CONCLUSIONS: Veterinarians can recommend the administration of these oral NSAIDs prior to obtaining blood for PRP and APS provided a single-day washout period is instituted.
摘要：
目的：观察长期口服NSAIDs保泰松和氟昔布对富含血小板血浆（PRP）和自体蛋白溶液（APS）中细胞因子和生长因子浓度的影响。
方法：6个成人大学拥有的马。
方法：马随机接受苯基丁酮（1克，口头,q12小时)或氟考昔(57毫克，口头,q24h)持续6天。在施用NSAID之前和在7天(NSAID停止后1天)获得血液并处理用于APS(Pro-Stride)和PRP(Restigen)。马经历了两周的淘汰期，在14天和21天获得血液。用交叉设计重复该方案。分析PRP和APS的血小板浓度，白细胞,和几种细胞因子(IL-1β，IL-10，IL-6，IL-8和肿瘤坏死因子-α）和生长因子（PDGF，FGF-2和TGF-β1）使用免疫测定法。评估血浆的药物浓度。
结果：在延长NSAIDs给药之前或之后，生长因子和细胞因子的浓度没有显著差异。与APS相比，PRP中的白细胞和血小板浓度存在显着差异，在第7天的时间点（T）中的白细胞浓度较高（苯基butazone），在T0（菲考昔布）和在T7（苯基butazone）的APS中的血小板浓度较高。
结论：兽医可以推荐在获得用于PRP和APS的血液之前给予这些口服NSAIDs，前提是建立一天的洗脱期。
方法：6个成人大学拥有的马。
方法：马随机接受苯基丁酮（1克，口头,q12小时)或氟考昔(57毫克，口头,q24h)持续6天。在施用NSAID之前和在7天(NSAID停止后1天)获得血液并处理用于APS(Pro-Stride)和PRP(Restigen)。马经历了两周的淘汰期，在14天和21天获得血液。用交叉设计重复该方案。分析PRP和APS的血小板浓度，白细胞,和几种细胞因子(IL-1β，IL-10，IL-6，IL-8和肿瘤坏死因子-α）和生长因子（PDGF，FGF-2和TGF-β1）使用免疫测定法。评估血浆的药物浓度。
结果：在延长NSAIDs给药之前或之后，生长因子和细胞因子的浓度没有显著差异。与APS相比，PRP中的白细胞和血小板浓度存在显着差异，在第7天的时间点（T）中的白细胞浓度较高（苯基butazone），在T0（菲考昔布）和在T7（苯基butazone）的APS中的血小板浓度较高。
结论：兽医可以推荐在获得用于PRP和APS的血液之前给予这些口服NSAIDs，前提是建立一天的洗脱期。
