Abstract：
The effects of lycopene (LP) on macrophage immune responses were evaluated in this study. Compared with the control treatment, LP treatment significantly increased cell vitality, phagocytic activity, and chemokine production in RAW264.7 cells. Additionally, compared with the control treatment, 4 μM LP treatment significantly activated autophagy, enhanced mitochondrial membrane potential, and upregulated receptor-interacting protein kinase 1 (RIPK1), while necrostatin-1 significantly reversed these effects of LP. Furthermore, compared with that in the control group, RIPK1 was significantly upregulated in the 4 μM LP and 4 μM LP + spautin-1 groups, whereas p-mTOR levels were reduced. More importantly, compared with that in the control group, p62 was significantly downregulated, and Beclin1, LC3-II, and Atg7 were upregulated in the 4 μM LP group, while spautin-1 significantly reversed these effects of LP. These results confirm that LP activates the mTOR/Beclin1/LC3/p62 autophagy signaling pathway through RIPK1, thereby enhancing the immune response of macrophages.
摘要：
在这项研究中评估了番茄红素（LP）对巨噬细胞免疫反应的影响。与对照治疗相比，LP处理显著增加细胞活力，吞噬活性，以及RAW264.7细胞中趋化因子的产生。此外,与对照治疗相比，4μMLP处理显著激活自噬，增强线粒体膜电位，和上调的受体相互作用蛋白激酶1（RIPK1），而necrostatin-1显著逆转了LP的这些作用。此外，与对照组相比，RIPK1在4μMLP和4μMLP+spauthin-1组中显著上调,而p-mTOR水平降低。更重要的是,与对照组相比，p62显著下调，和Beclin1,LC3-II,和Atg7在4μMLP组中上调，而spautin-1可显着逆转LP的这些作用。这些结果证实LP通过RIPK1激活mTOR/Beclin1/LC3/p62自噬信号通路,从而增强巨噬细胞的免疫应答。
