Abstract：
The emergence of zoonotic viruses like severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), and SARS-CoV-2 have significantly impacted global health and economy. The discovery of other viruses in wildlife reservoir species present a threat for future emergence in humans and animals. Therefore, assays that are less reliant on virus-specific information, such as neutralization assays, are crucial to rapidly develop diagnostics, understand virus replication and pathogenicity, and assess the efficacy of therapeutics against newly emerging viruses. Here, we describe the discontinuous median tissue culture infectious dose 50 (TCID50) assay to quantitatively determine the titer of any virus that can produce a visible cytopathic effect in infected cells.
摘要：
人畜共患病毒如严重急性呼吸道综合症冠状病毒（SARS-CoV）的出现，中东呼吸综合征冠状病毒（MERS-CoV），SARS-CoV-2对全球健康和经济产生了重大影响。在野生动物水库物种中发现其他病毒对人类和动物的未来出现构成威胁。因此,不太依赖病毒特异性信息的检测方法，如中和测定，对快速发展诊断至关重要，了解病毒复制和致病性，并评估治疗对新出现的病毒的疗效。这里,我们描述了不连续的中位组织培养感染剂量50（TCID50）测定法，以定量确定可以在感染细胞中产生可见的细胞病变效应的任何病毒的滴度。
